Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	50,280	T→G	F153C (TTT→TGT)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	50,280	0	T	G	100.0%	46.5 / NA	13	F153C (TTT→TGT)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/9);  total (4/9)

TTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCC  >  NZ_CP009273/50194‑50364                                                                                       |                                                                                     tttCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTGTga                                                                                   >  2:176318/1‑90 (MQ=255)             cGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTGTGAGATTCTGGAgcg                                                                       <  1:448008/90‑1 (MQ=255)               aGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTGTGAGATTCTGGAgcggc                                                                     <  2:520153/90‑1 (MQ=255)                  cGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTGTGAGATTCTGGAGCGGCGGt                                                                  <  1:714598/90‑1 (MQ=255)                         cTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTGTGAGATTCTGGAGCGGCGGTAATTTTg                                                           >  2:607674/1‑90 (MQ=255)                                  ggTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTGTGAGATTCTGGAGCGGCGGGAATTTTTTTTAGAAtt                                                  >  1:163992/1‑90 (MQ=255)                                        cAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGc                                            >  2:708597/1‑90 (MQ=255)                                                          tGATGCGCAGAACTCTCACAGCTATTGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGc                          <  1:598108/90‑1 (MQ=255)                                                                gcAGAACTCTCACAGCTATTGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTcg                    <  2:290385/90‑1 (MQ=255)                                                                         tcACAGCTATTGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATc           <  1:111977/90‑1 (MQ=255)                                                                                  ttGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc  <  1:708597/90‑1 (MQ=255)                                                                                  ttGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc  <  2:262281/90‑1 (MQ=255)                                                                                  ttGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc  <  2:82371/90‑1 (MQ=255)                                                                                       |                                                                                     TTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCC  >  NZ_CP009273/50194‑50364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑