Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	481,310	A→T	T32S (ACC→TCC)	acrR →	multidrug efflux transporter transcriptional repressor AcrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	481,310	0	A	T	100.0%	118.1 / NA	31	T32S (ACC→TCC)	acrR	multidrug efflux transporter transcriptional repressor AcrR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/15);  total (16/15)

GAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTC  >  NZ_CP009273/481224‑481393                                                                                       |                                                                                    gAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATcctcct                                                                                  >  2:284124/1‑90 (MQ=255) gAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATcctcct                                                                                  >  2:136440/1‑90 (MQ=255)    aaaCCAAACAAGAAGCCCAAGAAACGCGCCAACACATCCTCTATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGc                                                                               <  1:469734/90‑1 (MQ=255)             aagaagCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCgaga                                                                      <  2:88380/90‑1 (MQ=255)                aagCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTg                                                                   >  2:76928/1‑90 (MQ=255)                               gcCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCg                                                    <  2:99348/90‑1 (MQ=255)                                      caTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcg                                             >  2:186012/1‑90 (MQ=255)                                       aTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcgc                                            <  1:136440/90‑1 (MQ=255)                                       aTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcgc                                            <  1:239460/90‑1 (MQ=255)                                       aTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcgc                                            <  2:26185/90‑1 (MQ=255)                                       aTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcgc                                            <  2:156178/90‑1 (MQ=255)                                       aTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcgc                                            <  1:76928/90‑1 (MQ=255)                                       aTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcgc                                            <  1:511246/90‑1 (MQ=255)                                        tCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcgcg                                           >  2:203403/1‑90 (MQ=255)                                         ccTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCgg                                          <  1:462821/90‑1 (MQ=255)                                                  ggCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTa                                 >  1:327676/1‑90 (MQ=255)                                                  ggCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTa                                 >  2:146869/1‑90 (MQ=255)                                                        aCGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCa                           >  2:4000/1‑90 (MQ=255)                                                        aCGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCa                           >  1:318396/1‑90 (MQ=255)                                                        aCGTCTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCa                           <  1:8822/90‑1 (MQ=255)                                                            cTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcgc                                            <  1:497864/69‑1 (MQ=255)                                                            cTTTTCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcgc                                            >  2:497864/1‑69 (MQ=255)                                                             ttttCTCACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTa                      >  1:471776/1‑90 (MQ=255)                                                                tctcACAGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAg                   >  2:134987/1‑90 (MQ=255)                                                                    acaGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACaa               >  1:97941/1‑90 (MQ=255)                                                                     cagcagGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAg              >  2:389002/1‑90 (MQ=255)                                                                     cagcagGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAg              >  2:562665/1‑90 (MQ=255)                                                                          gggggTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGa         >  2:339903/1‑90 (MQ=255)                                                                            gggTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGAtt       <  1:562665/90‑1 (MQ=255)                                                                            gggTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGAtt       <  2:61298/90‑1 (MQ=255)                                                                               tATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGt    <  1:389002/90‑1 (MQ=255)                                                                                 tcatcCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTc  >  1:628532/1‑90 (MQ=255)                                                                                 tcatcCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTc  >  2:703958/1‑90 (MQ=255)                                                                                       |                                                                                    GAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTC  >  NZ_CP009273/481224‑481393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑