Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	768,739	C→T	A81V (GCT→GTT)	cydB →	cytochrome d ubiquinol oxidase subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	768,739	0	C	T	100.0%	68.8 / NA	19	A81V (GCT→GTT)	cydB	cytochrome d ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/6);  total (13/6)

ACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGCTGCGTTCTCCGGCTTCTATGTGGCGATGATCCTCGTGCTGGCGTCTTTGTTCTTCCGTCCGGTCGGTTTTGACTACCGCTCC  >  NZ_CP009273/768662‑768821                                                                              |                                                                                   acacTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGTTGCGTTCTCCgg                                                                        >  2:499570/1‑90 (MQ=255)      gggACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCt                                                                   >  2:227832/1‑90 (MQ=255)      gggACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCt                                                                   >  1:682291/1‑90 (MQ=255)           ggTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCTAtgtg                                                              >  1:213462/1‑90 (MQ=255)           ggTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCTAtgtg                                                              >  1:412552/1‑90 (MQ=255)            gTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCTATGTgg                                                             >  1:354865/1‑90 (MQ=255)            gTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCTATGTgg                                                             >  1:570585/1‑90 (MQ=255)                  aGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCTATGTGGCgatga                                                       >  1:541099/1‑90 (MQ=255)                            gATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCTATGTGGCGATGATCCTCGTGCt                                             >  1:16591/1‑90 (MQ=255)                                   gcgggcggCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCTATGTGGCGATGATCCTCGTGCTGGCGTCt                                      >  1:571752/1‑90 (MQ=255)                                                    tgctgcCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCTATGTGGCGATGATCCTCGTGCTGGCGTc                                       >  2:102934/1‑72 (MQ=255)                                                    tgctgcCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCTATGTGGCGATGATCCTCGTGCTGGCGTc                                       <  1:102934/72‑1 (MQ=255)                                                     gctgcCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCTATGTGGCGATGATCCTCGTGCTGGCGTCTTTGTTCTTCCGTCcggtc                    <  1:180279/90‑1 (MQ=255)                                                     gctgcCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCTATGTGGCGATGATCCTCGTGCTGGCGTCTTTGTTCTTCCGTCcggtc                    <  2:570585/90‑1 (MQ=255)                                                       tgcCTGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCTATGTGGCGATGATCCTCGTGCTGGCGTCTTTGTTCTTCCGTCcggtcgg                  <  2:213462/90‑1 (MQ=255)                                                           tGGCCGATGGTCTATGCCGTTGCGTTCTCCGGCTTCTATGTGGCGATGATCCTCGTGCTGGCGTCTTTGTTCTTCCGTCCGGTCGGtttt              >  2:233749/1‑90 (MQ=255)                                                                  tGGTCTATGCCGTTGCGTTCTCCGGCTTCTATGTGGCGATGATCCTCGTGCTGGCGTCTTTGTTCTTCCGTCCGGTCGGTTTTGACTAcc       <  1:409112/90‑1 (MQ=255)                                                                  tGGTCTATGCCGTTGCGTTCTCCGGCTTCTATGTGGCGATGATCCTCGTGCTGGCGTCTTTGTTCTTCCGTCCGGTCGGTTTTGACTAcc       <  2:16591/90‑1 (MQ=255)                                                                       tATGCCGTTGCGTTCTCCGGCTTCTATGTGGCGATGATCCTCGTGCTGGCGTCTTTGTTCTTCCGTCCGGTCGGTTTTGACTACCGCTcc  >  1:26087/1‑90 (MQ=255)                                                                              |                                                                                   ACACTGGGACGGTAACCAGGTTTGGCTGATCACCGCGGGCGGCGCACTCTTTGCTGCCTGGCCGATGGTCTATGCCGCTGCGTTCTCCGGCTTCTATGTGGCGATGATCCTCGTGCTGGCGTCTTTGTTCTTCCGTCCGGTCGGTTTTGACTACCGCTCC  >  NZ_CP009273/768662‑768821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑