| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 645,914 | 0 | A | G | 64.3% | 12.3 / 23.5 | 29 | T11T (ACT→ACC) ‡ | citE | citrate (pro‑3S)‑lyase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (2/8); new base G (18/0); total (20/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.67e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAG > NZ_CP009273/645835‑645996 | cATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGTTTTTCgtt > 1:213828/1‑90 (MQ=255) tCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTtgttgt > 1:247152/1‑90 (MQ=255) tCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCggttg > 1:111109/1‑86 (MQ=255) gTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTGTTGCAGCGa > 1:14824/1‑90 (MQ=255) aGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTGTTTCCGCGAAGCGGaaa > 2:1011155/1‑90 (MQ=255) aGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTGTTGTGGCGGAGCGGgaa > 2:439164/1‑90 (MQ=255) ggAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGAGTTTTTCGTTGTTGCAGCGAAACGGaaaa > 1:77924/1‑89 (MQ=255) aGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTGTTTCAGCGAAACGGAAAACa > 1:380961/1‑90 (MQ=255) aGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCCTTGTTGCAGCGAAGCGGgaaaaa > 1:865673/1‑87 (MQ=255) gTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTGTTGCAGCGGAACGGGAAACaa > 2:214405/1‑89 (MQ=255) gTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTGGTTGTTTCCGCGAAGCGGAaataat > 2:483045/1‑90 (MQ=255) ccATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAAtc < 2:850008/90‑1 (MQ=255) ccATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTGTTGCAGCGAAACGGGAAACATTTGccaac > 2:331409/1‑86 (MQ=255) aTCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTTTTGGAGGGAAGAGGGAAACATTGGGAAtccg > 1:420394/1‑89 (MQ=255) aTTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGc < 2:144439/90‑1 (MQ=255) ttGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCa < 1:924946/90‑1 (MQ=255) aCCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGcc < 2:493243/90‑1 (MQ=255) cAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTGTTTGAGCGAAACGGGAAAAAATGGGAAATCTCCCCAGGCTGAGGCgg > 2:575814/1‑90 (MQ=255) cAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTGTTGCAGCGAAGCGGAAAAAATTTGGAATCCTCCCCTGGCGGAGGCgg > 2:430317/1‑90 (MQ=255) cAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTTGTTTTTGCAGCGAAGCGGAAAACATTTGCAATCCCCCCCTGGCAGAGcccg > 1:1100321/1‑88 (MQ=255) cAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTACGTTGTTGTGGGGGAGGGGAAATCATTGGGGAACCTCCCCCGGCAGAGGCgg > 2:196187/1‑90 (MQ=255) acaaacaaCATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTTCAGCGGAACGGGAATCATTGGCAATCCTCCCCTGGCAGAGGCGGGGTAc > 1:124159/1‑90 (MQ=255) acaTGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGgc < 2:964165/90‑1 (MQ=255) acaTGCTGCGGCGGGGGGGGGGTTTTCGTTTTTGCAGCGAAACGGGAAACATTTGCAATCCCCCCCCGGCAGAGCCGGGGGACCGCCggg > 1:969081/1‑89 (MQ=255) acaTGCTGCGGCGGGGGCGAGGTTTTCGTTGTTGCAGCGAAGCGGGAAACATTTGCAAACCTCCCCTGGCAGAGGCGGGGTACCGCCGgc > 1:344170/1‑90 (MQ=255) aTGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGgcgc < 1:989610/90‑1 (MQ=255) cggGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCcagca < 2:1100321/90‑1 (MQ=255) gggTGCGCGTTTTAAGTTGTTGAAGCGAAGCGGAAATCTTTGGCAATCCTCCCATGGCAGAGCCGGTATCCCGCTGGCGCGCGCcagcag < 2:835003/90‑1 (MQ=255) gggTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCcagcag < 2:865673/90‑1 (MQ=255) | CATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAG > NZ_CP009273/645835‑645996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |