| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 687,303 | 0 | A | G | 54.5% | 20.6 / 43.0 | 33 | intergenic (‑63/+27) | corC/ybeY | CNNM family magnesium/cobalt transport protein CorC/rRNA maturation RNase YbeY |
| Reads supporting (aligned to +/- strand): ref base A (3/12); new base G (18/0); total (21/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-06 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGA > NZ_CP009273/687217‑687391 | actGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCgg > 1:12543/1‑89 (MQ=255) ctGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCggc > 1:163738/1‑88 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGTCTg > 2:58002/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 2:357573/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 1:1112746/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTg > 2:1088521/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTg > 1:529560/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTg > 1:210202/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTg > 1:159169/1‑90 (MQ=255) gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGcc < 1:357573/90‑1 (MQ=255) aaTTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGGCTGcccgg > 2:1000756/1‑87 (MQ=255) aaTTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTTccgcg > 2:875780/1‑87 (MQ=255) tcgtcgCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGGCTGCCAGTCaaaa > 2:27918/1‑89 (MQ=255) tcgtcgCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCCGTCAAAt > 1:1119482/1‑90 (MQ=255) tcgtcgCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGGCAAAt > 2:452009/1‑90 (MQ=255) tGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGACAGTCAAATGCCTGGCaaa < 1:114887/90‑1 (MQ=255) tGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCCCTCAAAAGCCTGGCCAATTAttttttt > 2:951132/1‑89 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAAAGCCTGGGAAAATATTTTTTTTTGGc > 2:103877/1‑90 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc < 1:791765/90‑1 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGGCTGCCCGTCAAAAGCCTGGCAAAATATTCTTTCTCGGc > 1:1021520/1‑90 (MQ=255) gttagttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGTCAAAAGCCTGGCAAAATATTTTTTTTCCGCAATGTa > 2:453400/1‑90 (MQ=255) ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTAc < 2:159169/90‑1 (MQ=255) agttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCAGTCAAATGCCTGGCAAATTATTTTTTTTCGGCAATGTACgg > 1:635130/1‑90 (MQ=255) agttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCAGTCAAAAGCCTGGCAAAATATTTTTTCTCGGCAATGTACgg > 1:890683/1‑90 (MQ=255) taATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 2:434735/90‑1 (MQ=255) taATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 2:43880/90‑1 (MQ=255) taATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 1:504710/90‑1 (MQ=255) cAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCa < 2:1021520/90‑1 (MQ=255) gCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGccc < 1:1000756/90‑1 (MQ=255) gtcgtTAATCACCAACGGCGGGGACGTCTGCCCGTCAAAAGCCTGGCAAATTATTTTTTTTCGGCAATGTACGGATCCTCATAGCCCaga > 1:769315/1‑90 (MQ=255) cgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagc < 1:103877/90‑1 (MQ=255) aaCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAAtct < 1:1039561/90‑1 (MQ=255) gACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGa < 1:843863/90‑1 (MQ=255) | ACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGA > NZ_CP009273/687217‑687391 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |