| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,955 | 0 | T | G | 55.6% | 27.2 / 37.6 | 27 | T495P (ACC→CCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/12); new base G (15/0); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.75e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.62e-06 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGC > NZ_CP009273/822871‑823037 | cTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGgggggggg > 2:39604/1‑90 (MQ=255) aTCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGgggggggggggg > 2:104960/1‑88 (MQ=255) aTCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAggggggc > 1:813372/1‑88 (MQ=255) tAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGc < 2:324606/90‑1 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGAAg > 2:358795/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAg > 1:160130/1‑90 (MQ=255) gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGTCGGGtc > 2:209108/1‑90 (MQ=255) tCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGAAGTCGGGttgtcgg > 1:246220/1‑89 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCCGAAGGCGGTTTGTCGAGaaaa > 1:512839/1‑89 (MQ=255) gCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGAAGTCGGGTCGTCGAGAAAAAGAAt > 1:310949/1‑90 (MQ=255) ccTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGGAAGCCCGGAGGCGGGTCGTCGAGAAACAGaaag > 2:698826/1‑88 (MQ=255) tttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGTCGTCGCGAAACAGAAAGTc > 1:661896/1‑90 (MQ=255) ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAGCGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc < 2:1089292/90‑1 (MQ=255) ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc < 1:104960/90‑1 (MQ=255) acagctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc < 2:635770/86‑1 (MQ=255) ctctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCgg < 1:594950/90‑1 (MQ=255) ctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGtt < 2:677528/90‑1 (MQ=255) ccATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCAt < 1:135592/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 1:21970/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGAAGGCGGGTCGTCGAGAAAAAAAAAGGCCGGGTGATGc > 2:554287/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCGACGCCGGAAGTCGGGTCGTCGGGAAAAAGAATGTCCGGGGCATgg > 2:1062444/1‑89 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGc > 2:280796/1‑90 (MQ=255) cTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATc < 1:280796/90‑1 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg < 1:602910/90‑1 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACCCCGGGAGGCGGGTCGTCGAGAAAAAGAAAGGCCGGGTCATGCATCAGCGGACaag > 2:934680/1‑88 (MQ=255) gCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCGAACAgg > 1:132913/1‑90 (MQ=255) aGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgcc < 1:1114260/90‑1 (MQ=255) aCGGCGGGGGGGGGGGGGAACGCCGGGAGGCGGGTCGGCGAGAAAAAAAAAGGCCGGGTCGTGCAACCGCGGACAGGCCAGCGCccgccg > 2:677713/1‑90 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:554287/90‑1 (MQ=255) | CTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGC > NZ_CP009273/822871‑823037 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |