| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,819,313 | 0 | G | A | 63.2% | ‑5.7 / 14.3 | 19 | intergenic (‑119/+84) | ves/spy | environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy |
| Reads supporting (aligned to +/- strand): ref base G (6/1); new base A (0/12); total (6/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.58e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGA > NZ_CP009273/1819238‑1819392 | cgggggATGTTTATCGGCAACTGGGGTGATTTACTTTAGTGGTGATCAGGTTTTTTTTACAACTGTTGGAGAAGAAAAAAAAAAAccgcc < 2:421376/86‑1 (MQ=255)ccGTGGTTGTTTATCGGCAACTGGGGTAATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTGAGAAGAAAAAAAAAAAccgcc < 2:28771/90‑1 (MQ=255)ccGGGGATGTTTACCGGCACCTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAAccgcc < 2:307031/90‑1 (MQ=255) tGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGAt > 1:307031/1‑90 (MQ=255) ggATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATc > 2:610543/1‑90 (MQ=255) ttATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTcc > 1:588453/1‑90 (MQ=255) ttATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTcc > 2:1050138/1‑90 (MQ=255) tCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGTTTTTTTTTACAACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCAcc < 2:61216/90‑1 (MQ=255) accTCGGGTGATTTACGTTAGTGGTGATCGGTTTTTTTTTACAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAtt < 2:141250/88‑1 (MQ=255) aaCTGGGGTTTTTTACTTTAGTGGTGATCAGGTTTTTTTTACAATTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAtt < 1:610543/90‑1 (MQ=255) aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTa > 1:61216/1‑90 (MQ=255) cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTAc < 1:280493/90‑1 (MQ=255) gtattttACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 2:236861/86‑1 (MQ=255) gTGATTTACTTTTGTGGTTCTCAGGTTTTTTTTACAATTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 1:544536/90‑1 (MQ=255) ttACGTTAGTGGTGATCGGGTTTTTTTTACAACTGTTTGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg < 1:1001482/90‑1 (MQ=255) ttACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg < 1:389796/90‑1 (MQ=255) ggCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTc > 2:455448/1‑90 (MQ=255) ttttttACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTaa < 1:641543/90‑1 (MQ=255) tttGAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGa < 2:1069427/90‑1 (MQ=255) | CCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGA > NZ_CP009273/1819238‑1819392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |