| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,571 | 0 | A | G | 63.2% | 1.6 / 18.7 | 19 | I83V (ATT→GTT) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base A (0/7); new base G (12/0); total (12/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.98e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCC > NZ_CP009273/2089486‑2089653 | ttCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGttta > 2:164070/1‑88 (MQ=255)ttCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTaa > 1:390042/1‑90 (MQ=255) tCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAg < 1:603444/90‑1 (MQ=255) gCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGtctc < 2:229540/90‑1 (MQ=255) aGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTTAGTCTCTTGGAGGTTTCGCGaa > 2:910145/1‑90 (MQ=255) aGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGaa < 1:663183/90‑1 (MQ=255) aGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAATTTCTGGGAGATGCCGCGaa > 1:600223/1‑90 (MQ=255) aaaGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGGTTTTGGAGAAGCCGCGaaaaatt > 2:699929/1‑87 (MQ=255) gggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGACTCTGGAAGATGCCGCGGAAAATTTTTCCt > 2:202003/1‑90 (MQ=255) tctcGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGATGCCGCGAAAAATTTTTTCTTTTgc > 1:596749/1‑90 (MQ=255) tcGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGgcgc < 2:620192/90‑1 (MQ=255) tcGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAATCTTTGGAAGATGGCGCGAAAAATCTTTTCTTTggggg > 1:901266/1‑87 (MQ=255) gATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCGCGAAAATTTTTTTCTTTTGGGGGGGTAAAAttttcct > 1:43518/1‑86 (MQ=255) tCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAGTCTCTGGAAAATGCCGCGAAAAATCTTTTCTTTTGGGCGGGTAAAAATTtcatca > 1:583721/1‑90 (MQ=255) gACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAAc < 1:125439/90‑1 (MQ=255) gTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCCCGAAAATTCTTTTCTTTTGCGCGGGTAAAAATTTCATCAACTCTCCTgc > 2:408906/1‑90 (MQ=255) gtgtgGCGGGTGGGGTTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTTCTTTTGCGCGGGTAAAAATTTCATCAACTCTCCTGCGCTgg > 2:1121989/1‑90 (MQ=255) tgtgGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGc < 1:143117/90‑1 (MQ=255) gggTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGAccc < 2:202855/90‑1 (MQ=255) | TTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCC > NZ_CP009273/2089486‑2089653 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |