| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,436,058 | 0 | T | G | 52.9% | 14.4 / 18.1 | 17 | G272G (GGT→GGG) | mnmC | bifunctional tRNA (5‑methylaminomethyl‑2‑thiouridine)(34)‑methyltransferase MnmD/FAD‑dependent 5‑carboxymethylaminomethyl‑2‑thiouridine(34) oxidoreductase MnmC |
| Reads supporting (aligned to +/- strand): ref base T (2/6); new base G (9/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGG > NZ_CP009273/2435985‑2436146 | tACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTTCCcgcgcgg > 1:531769/1‑89 (MQ=255) gCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTGCCCGCGCgttgtt > 1:771632/1‑90 (MQ=255) cTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGTATTGCCAGCGCggtgttt > 1:50036/1‑89 (MQ=255) cTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTGCCAGCGCggtgttt > 1:29882/1‑89 (MQ=255) ccccTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTGCCAGCGCGGTGTTTTc > 1:968109/1‑90 (MQ=255) gCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTGCCCGCGCGGTGTTTTCGCTgg > 2:57309/1‑90 (MQ=255) gcgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTTCCCGCGCGGTGTTGTTGCTGGGGCTa > 1:635395/1‑90 (MQ=255) gcgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTTCCCGCGCGGTGGTGTTGCTGGCGCTa > 2:902824/1‑90 (MQ=255) gcgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTGCCAGCGCGGTGTTGTCGCTGGCGCTa > 1:318400/1‑90 (MQ=255) aCGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGATTTCCAGCGCGTTGTTTTCGCTGGGGCTATTACGGCGCGGGTGGGAg > 1:973490/1‑90 (MQ=255) gcagcagcaAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTaa < 1:450060/90‑1 (MQ=255) aaCGGGAAGCGGCGATTATCGGCGGTGGGATTGCCCGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGGAGGGAAAGCCTtttt > 1:914096/1‑90 (MQ=255) ggAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTgcgc < 1:243680/90‑1 (MQ=255) cggcgATTATCGGCGGTGGTATTGCCAGTGCGTTGTTGTCGCTGGCGCTATTCCGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATg < 2:973490/90‑1 (MQ=255) gcgATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAg < 2:235086/90‑1 (MQ=255) tATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGcccc < 1:542503/90‑1 (MQ=255) gcggTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCAc < 2:968109/90‑1 (MQ=255) gtggtATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTgg < 2:976755/90‑1 (MQ=255) | TACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGG > NZ_CP009273/2435985‑2436146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |