Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,055,6790AC52.2% 48.1 / 71.9 46T491P (ACC→CCC) scpAmethylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base A (21/1);  new base C (0/24);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 3.17e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-09
Rejected as consensus: Frequency below/above cutoff threshold.

TGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAAT  >  NZ_CP009273/3055590‑3055768
                                                                                         |                                                                                         
tGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCa                                                                                           <  1:111123/90‑1 (MQ=255)
tGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCa                                                                                           <  2:325165/90‑1 (MQ=255)
  gATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCAcc                                                                                         >  2:745877/1‑90 (MQ=255)
      aCGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGtg                                                                                     >  2:589218/1‑90 (MQ=255)
         aaGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGtgatg                                                                                  >  1:158606/1‑90 (MQ=255)
             cGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATgc                                                                              <  1:589218/90‑1 (MQ=255)
                     aTGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTaa                                                                      >  1:1010043/1‑90 (MQ=255)
                         tcTTGAGAACGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCCCCCGTGATGATGCCGCCGTAAccgc                                                                  <  1:53851/89‑1 (MQ=255)
                            tGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACcgccgc                                                               >  2:848086/1‑90 (MQ=255)
                             gagaTCGACAACGTGATGGGGCGAAACGAGAAAATTGCTTCGCTGGAACGCATTCGCGCCCCCCGTGATGATGCCGCCGTAACCGCcgcg                                                              <  2:468968/90‑1 (MQ=255)
                               gaTCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGtt                                                            >  2:904035/1‑90 (MQ=255)
                               gaTCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGtt                                                            >  1:419477/1‑90 (MQ=255)
                               gaTCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGtt                                                            >  1:911160/1‑90 (MQ=255)
                                 tCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGa                                                          >  1:845708/1‑90 (MQ=255)
                                  cGACACCGTGAGGGTGCGAAACGAGAAAATTCCTCCGCTGGAACGCATCCGCCCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGaa                                                         <  2:146782/90‑1 (MQ=255)
                                  cGACAAGGTGATGGTGCGTAACGAGAAAATTGCTTCGCTGGAACGCATTCGCGCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGaa                                                         <  1:927953/90‑1 (MQ=255)
                                   gACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAAc                                                        >  1:757393/1‑90 (MQ=255)
                                   gACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAAc                                                        >  2:943723/1‑90 (MQ=255)
                                   gACAACGTGAGGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAAc                                                        <  1:826237/90‑1 (MQ=255)
                                    aCAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACg                                                       >  2:386608/1‑90 (MQ=255)
                                       aCGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGccc                                                    <  1:384427/90‑1 (MQ=255)
                                               gTGCGTAACGAGAAAATTGCTCCGCGGGACCCCATCCGCCCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAc                                            <  2:911160/90‑1 (MQ=255)
                                                 gCGTAGCGGGCAATTTTCTCCGCTGAAACGCATCCGCCCCCCCCGTGATGATCCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcgc                                          <  2:740462/90‑1 (MQ=255)
                                                 gCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcgc                                          >  2:460748/1‑90 (MQ=255)
                                                 gCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcgc                                          >  1:174057/1‑90 (MQ=255)
                                                 gCGTAACGAGAAATTTGCTCCGCGGGACCCCATCCGCCCCCCCCGTGATGAGCCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcgc                                          <  2:763995/90‑1 (MQ=255)
                                                   gTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcgccg                                        >  1:466832/1‑90 (MQ=255)
                                                   gTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcgccg                                        >  2:749102/1‑90 (MQ=255)
                                                      aCGAGCAATTTGCTTCGCTGGAACGCATTCGCCCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGcac                                     <  2:381633/90‑1 (MQ=255)
                                                      aCGAGCAATTTGCTCCGCGGGACCGCTTTCGCCCCCCCCGTGATGATCCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGcac                                     <  2:174057/90‑1 (MQ=255)
                                                      aCGAGCAAATTGCTTCGCTGGACCGCATCCGCCCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGcac                                     <  2:757393/90‑1 (MQ=255)
                                                      aCGAGCAAATTGCTTCGCTGGACCGCATCCGCCCCCCCCGGGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGcac                                     <  2:22936/90‑1 (MQ=255)
                                                      aCGAGAAATTTCCTCCGCGGGACCGCATCCGCCCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGcac                                     <  2:160885/90‑1 (MQ=255)
                                                        gAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAg                                   >  2:865234/1‑90 (MQ=255)
                                                        gAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAg                                   >  1:729255/1‑90 (MQ=255)
                                                         aGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGc                                  >  1:1109797/1‑90 (MQ=255)
                                                         aGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGc                                  >  2:243468/1‑90 (MQ=255)
                                                           cAACTTGCTCCGCTGGACCGCTTCCGCGCCCCCCGGGATGAGGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCAt                                <  2:268816/90‑1 (MQ=255)
                                                           cAAATTGCTTCGCTGGAACGCATCCGCCCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCAt                                <  1:497161/90‑1 (MQ=255)
                                                           cAAATTGCTCCCCCGGAACGCATCCCCCCCCCCCGTGAGGACCCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCAt                                <  2:826300/90‑1 (MQ=255)
                                                           aaatttGCTCCGCGGGAACGCTTCCGCCCCCCCCGTGAGGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCAt                                <  2:1109797/86‑1 (MQ=255)
                                                                     cgcgGGACCGCATTCGCGCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAAcc                      <  1:291828/90‑1 (MQ=255)
                                                                     cGCTGGAACGCATTCGCGCCCCCGGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAAcc                      <  1:469920/90‑1 (MQ=255)
                                                                      gCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACct                     >  2:510722/1‑90 (MQ=255)
                                                                                 ttCGCCCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGAGTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCg          <  1:372488/90‑1 (MQ=255)
                                                                                 tccgccCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCg          <  1:904035/88‑1 (MQ=255)
                                                                                 tccgccCCCCCCGTGAGGACCCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCg          <  2:1010043/88‑1 (MQ=255)
                                                                                   cgcgCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCt        <  1:390923/90‑1 (MQ=255)
                                                                                   cgccccgcccGTAAGGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCt        <  1:262826/90‑1 (MQ=255)
                                                                                         aCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAAt  >  1:915241/1‑90 (MQ=255)
                                                                                         aCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAAt  >  1:241875/1‑90 (MQ=255)
                                                                                         |                                                                                         
TGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAAT  >  NZ_CP009273/3055590‑3055768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: