Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,910	T→G	W30G (TGG→GGG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,910	0	T	G	100.0%	172.8 / NA	48	W30G (TGG→GGG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/25);  total (23/25)

TCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49827‑49996                                                                                    |                                                                                       tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTa                                                                                  <  2:839457/90‑1 (MQ=255)   aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTaaa                                                                                >  2:297054/1‑90 (MQ=255)         aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                          <  2:505217/90‑1 (MQ=255)         aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                          <  1:797903/90‑1 (MQ=255)         aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                          <  2:772242/90‑1 (MQ=255)         aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACCCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGGTTAAACGCAac                                                                          >  1:771836/1‑90 (MQ=255)            gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACAcc                                                                       >  2:503695/1‑90 (MQ=255)            gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGGTTAAACGCAACAcc                                                                       >  2:296698/1‑90 (MQ=255)              ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGGACCTGCCCGCCGATCTCGCCGGGTTTAAACGCAACACCtt                                                                     >  2:753334/1‑90 (MQ=255)              ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCtt                                                                     >  2:368513/1‑90 (MQ=255)              ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCtt                                                                     >  1:198461/1‑90 (MQ=255)              ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                                                      >  2:153019/1‑89 (MQ=255)              ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                                                      <  1:153019/89‑1 (MQ=255)              ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTACCTGCCGATCTCGCCGGGTTTAAACGCAACACCtt                                                                     >  1:268592/1‑90 (MQ=255)                  ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaat                                                                 <  1:880578/90‑1 (MQ=255)                  ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGGTTAAACGCAACACCTtaaat                                                                 >  1:596497/1‑90 (MQ=255)                    aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaataa                                                               <  1:368513/90‑1 (MQ=255)                    aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaataa                                                               <  2:1120717/90‑1 (MQ=255)                       ggTAGATCGCGTTATCGGCATGAAAAACGCCATGCCGTGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAcc                                                            <  2:110483/90‑1 (MQ=255)                             tCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGAt                                                      >  1:35895/1‑90 (MQ=255)                               gcgTTATCGGCATGGAAAACGCCATGCCGTGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                                    <  2:319335/90‑1 (MQ=255)                               gcgTTATCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGATCTCGCCGGGGTTAAACGCAACACCTTAAATAAACCCGTGGTTa                                                    >  1:838124/1‑90 (MQ=255)                                       ggCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                            >  2:259593/1‑90 (MQ=255)                                          aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                         <  1:203435/90‑1 (MQ=255)                                           tGGAAAACGCCATGCCGGGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATa                                        <  1:767384/90‑1 (MQ=255)                                              aaaaCGCCATGCCGGGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                                     <  1:503695/90‑1 (MQ=255)                                                  cGCCATGCCGTGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                                 <  2:435243/90‑1 (MQ=255)                                                  cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTa                                                                                  <  2:284388/41‑1 (MQ=255)                                                  cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTa                                                                                  >  1:284388/1‑41 (MQ=255)                                                  cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                          >  2:539735/1‑49 (MQ=255)                                                  cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                          <  1:539735/49‑1 (MQ=255)                                                  cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                                 >  2:103133/1‑90 (MQ=255)                                                      aTGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca                             >  2:681230/1‑90 (MQ=255)                                                         ccGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaataaa                                                              <  2:1030365/54‑1 (MQ=255)                                                         ccGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaataaa                                                              >  1:1030365/1‑54 (MQ=255)                                                           gTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                         >  2:888482/1‑73 (MQ=255)                                                           gTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                         <  1:888482/73‑1 (MQ=255)                                                             ggAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc                      <  1:297054/90‑1 (MQ=255)                                                                aCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc                   <  2:35895/90‑1 (MQ=255)                                                                   tgcctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                >  2:530587/1‑90 (MQ=255)                                                                   tgcctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                >  1:1033222/1‑90 (MQ=255)                                                                    gcctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGtt               >  1:593263/1‑90 (MQ=255)                                                                            aTCTCGCCGGGTTTAAACGCAACCCCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc       <  1:61957/90‑1 (MQ=255)                                                                            aTCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc       >  2:534104/1‑90 (MQ=255)                                                                                 gCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  2:198461/90‑1 (MQ=255)                                                                                 gCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  1:759428/90‑1 (MQ=255)                                                                                 gCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  1:639059/90‑1 (MQ=255)                                                                                 gCCGGGTTTAAACGCAACACCTTAAAAAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  1:278282/90‑1 (MQ=255)                                                                                    |                                                                                       TCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49827‑49996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑