| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 349,999 | 0 | T | G | 52.4% | 34.1 / 62.1 | 42 | V528G (GTG→GGG) | prpE | propionate‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (1/19); new base G (22/0); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-07 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAC > NZ_CP009273/349911‑350084 | aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTg < 1:553373/90‑1 (MQ=255) cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTTGGGGGGa > 2:44568/1‑90 (MQ=255) ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAg > 2:539307/1‑90 (MQ=255) ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGGGGGGGGGAAg > 2:487116/1‑90 (MQ=255) cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATgc < 1:48500/90‑1 (MQ=255) ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATgcgc < 1:555237/90‑1 (MQ=255) gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGAAAAAGgcgcc > 2:302544/1‑89 (MQ=255) ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGAGGCGGTGAAAagggg > 1:385317/1‑88 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAgggggc > 1:329891/1‑90 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAgggggg > 1:56975/1‑89 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAggtggg > 1:93106/1‑89 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGTTGGGGGGAAAGATGCGGTGAAAGGGCTgggggg > 1:399643/1‑89 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGGGCTGAAAGGGCAgggggg > 1:598869/1‑89 (MQ=255) cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggt < 1:487116/90‑1 (MQ=255) gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc < 1:434376/90‑1 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAAGGGAGGGGGCGGTGGCGGTTGTCAt > 1:211759/1‑90 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGTTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGGGGGGGGGGTTGTTAt > 2:452823/1‑90 (MQ=255) ccAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag < 2:581102/90‑1 (MQ=255) ccAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag < 1:368588/90‑1 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagag < 1:293467/90‑1 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAAGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAagag > 1:657867/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGTTGGGGGGGAAGATGCGCTGAAAGGGCGGGGGGGGGGGGGGGTTTTCATTCCGAAagag > 1:581102/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAAATGGGCTGGAAGGGGGGGGGGGGGGGGGGGTTGTCATTTCGAAaaag > 2:571057/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAAAAGCGCTGGAAGGGGAGGGGGGGGGGGGGGTTGTTATTTCGGAaaag > 2:368588/1‑90 (MQ=255) aTCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGCTGAAAGGGGGGGGGGGGGGGGGGGTTTTCATTCCGAAagagaggg > 2:364130/1‑88 (MQ=255) aTCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGTTTGTCATTCCGAAAGAGAGCg > 1:78263/1‑90 (MQ=255) aTCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAAAAGCGCTGAAAGGGGAGGGGGCGGGGGGGGTTTTGCTTCCGGAAAAGAGCg > 2:263613/1‑90 (MQ=255) aTCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAAAAGCGCTGAAAAGGGGGGGGGGGGGGGGGGTTGTTATTTCGGAAGAGAGCg > 2:620587/1‑90 (MQ=255) tCCGGGCGTTGCCGAAGTGGC‑GGGGGTTGGGGGGAAAGATGCGCTGGAAGGGGAGGGGGGGGGGGGGTTTGTCATTTCGAAAGAGAGGGa > 2:149544/1‑90 (MQ=255) ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:370445/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:571057/90‑1 (MQ=255) ccGGGCGGTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:399643/90‑1 (MQ=255) gggCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 1:641978/90‑1 (MQ=255) gggCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 1:44568/90‑1 (MQ=255) gggCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 2:598869/90‑1 (MQ=255) cGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCt > 1:499577/1‑90 (MQ=255) ttGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcgc < 2:56975/90‑1 (MQ=255) tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa < 1:620587/90‑1 (MQ=255) tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa < 1:539307/90‑1 (MQ=255) ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc < 1:149544/90‑1 (MQ=255) gggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt < 2:315996/74‑1 (MQ=255) gggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt > 1:315996/1‑74 (MQ=255) | AACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAC > NZ_CP009273/349911‑350084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |