| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 711,081 | 0 | A | C | 52.9% | 18.4 / 23.0 | 17 | T72P (ACC→CCC) | BW25113_RS03560 | lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (8/0); new base C (0/9); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAA > NZ_CP009273/710998‑711166 | gagGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACccgcc > 2:539145/1‑90 (MQ=255)gagGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACccgcc > 2:257804/1‑90 (MQ=255) tAAGGGGTAGCATTGCGGTAAATATTCCCCCTTCCCGGTGAAAAAAAAATTGCGGTGGTAATAACTTCCCTAAAAATCCCCGCCGCTGGc < 2:431705/90‑1 (MQ=255) gTAAATTTTGCCCCTTCCCGGTGAAAGAAAAATTGCCGTGGTGATAACTTACCTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAAc < 2:169830/90‑1 (MQ=255) ttGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAg > 1:169830/1‑90 (MQ=255) gccccTTTCCGGTGAAAGAAAAATTGCGGGGGTGAAAACTTACCTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGaa < 2:681520/87‑1 (MQ=255) gCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGaa > 2:640192/1‑90 (MQ=255) cagcTTCCCGGTGAAAAAAAAATTGCGGGGGTAAAAACTTACCTAAAAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAac < 2:486414/87‑1 (MQ=255) ccTTTCGGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTAATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAactt < 1:267352/90‑1 (MQ=255) tttCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAacttac > 1:78169/1‑90 (MQ=255) ggTGAAAGAAAAATGGCCGTGGTGATAACTTCCCTAATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATc < 1:622644/90‑1 (MQ=255) gaaaGAAAAATTGCGGGGGTAATAACTTACCTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTc < 2:1374/88‑1 (MQ=255) tAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCtctg > 1:486414/1‑87 (MQ=255) aGAAAAATTGCCGTGGTGATAACTTACCTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCata < 1:335621/90‑1 (MQ=255) ttGCCGTGGTGATAACTTCCCTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTc < 1:3411/90‑1 (MQ=255) tACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCg > 1:244833/1‑90 (MQ=255) aCCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCgg > 1:452992/1‑90 (MQ=255) ctaataatcCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGtt < 1:539145/87‑1 (MQ=255) taataCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTaaa > 2:486837/1‑90 (MQ=255) | GAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAA > NZ_CP009273/710998‑711166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |