| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 799,325 | 0 | G | T | 56.2% | 6.8 / 27.2 | 16 | V123L (GTG→TTG) | ybhJ | hydratase |
| Reads supporting (aligned to +/- strand): ref base G (1/6); new base T (9/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACC > NZ_CP009273/799244‑799414 | ctctGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCg < 2:367811/90‑1 (MQ=255) tctGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGGCTCCGc > 1:7718/1‑90 (MQ=255) gTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGGCCCCCCCTATTTCg > 1:517351/1‑90 (MQ=255) cggcggCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGt < 2:621971/90‑1 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGTCGGCCCAGCGTTATGGCGGGGTTTTTTTGTGTCCCCACATTACGGGCATTCCTCAATATTTgag > 2:584049/1‑88 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGGTTTTTTTTTCTCCCCCTATTTTGGGCCTCCCTCAatatatttg > 2:477951/1‑87 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTTGCTCCCCCTTTTTTGGGCATCCCTCAatataatcg > 2:556071/1‑86 (MQ=255) atgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTg < 1:477951/90‑1 (MQ=255) atgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTg < 1:556071/90‑1 (MQ=255) atgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGGCTCCCCCTATTTTGGGCATCCCTCAATATATTCGTg > 2:108476/1‑90 (MQ=255) atgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGGCCCCCCCTATTTCGGGCATCCCTCAATATATGCGTg > 1:345590/1‑90 (MQ=255) tttGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCCCCCCCTATTTCGGGGCTCCCTCAAAATATGCGTGGGGTGATgggg > 2:93523/1‑88 (MQ=255) tATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTca < 2:130105/54‑1 (MQ=255) tATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTca > 1:130105/1‑54 (MQ=255) tATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCggg < 1:351269/90‑1 (MQ=255) ggTATTTTTTTGCCTCCGCCTATTTCGGGCATCCATCAATATATGCGTGGGGTGATGGCCGGCGGGGGGGAAAAGATCCTCCGGGCAGag > 2:351269/1‑89 (MQ=255) gtgCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACAcc < 1:170037/90‑1 (MQ=255) | CTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACC > NZ_CP009273/799244‑799414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |