| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,963 | 0 | T | G | 60.0% | ‑4.0 / 12.9 | 15 | D492A (GAC→GCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAA > NZ_CP009273/822887‑823051 | ggtggtGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCGACGCCGGAAgg > 2:667456/1‑89 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGGCGGGTCGTCGAGAAAc > 2:247245/1‑90 (MQ=255) ctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGtt < 1:97620/90‑1 (MQ=255) ccATGCCGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCAt < 1:484093/90‑1 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGTTCGCCGAGAAAAAGGAAGTTCGGGTCATGCATCAg > 2:639406/1‑90 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGAAGGCGGGTCGTTGGGAAACAAAAAGTCCGGGTCTTTCATCAg > 2:89592/1‑90 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 2:554553/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 2:621229/90‑1 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGTTCGTAGAGAAAAAGAAAGTCCGGTTCCTGCATCAGCGAACAgg > 1:521627/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGGTCGTCGGGAAAAAAAATGTCCGGGTCATGCATCAGCGAACaag > 1:594991/1‑88 (MQ=255) aGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAGTCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCGGAAAgggc > 1:409329/1‑88 (MQ=255) aGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGGCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGCATCAGCGAACAgggc > 2:303687/1‑88 (MQ=255) aCGGCGGGGGGGGGGGGCAACGCCCGGAGGCGGGTCGTCGGGAAAAAAAAAGTCCGGGTCATGCATCAGCGAACAGGCCAGCGCCAGCCg > 2:669905/1‑90 (MQ=255) gAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAACCAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaaa < 2:409329/90‑1 (MQ=255) gTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTaa > 1:71487/1‑90 (MQ=255) | GGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAA > NZ_CP009273/822887‑823051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |