| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,319,929 | 0 | T | G | 52.6% | 8.4 / 25.7 | 19 | G114G (GGT→GGG) | atoB | acetyl‑CoA acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/8); new base G (10/0); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAA > NZ_CP009273/2319849‑2320017 | tgtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGgaaaa > 2:248634/1‑89 (MQ=255) tgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAaaat > 2:354283/1‑90 (MQ=255) ggTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATg < 2:115152/90‑1 (MQ=255) ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGccc < 2:485805/90‑1 (MQ=255) gtgtGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGGTTTGGCCCCcact > 1:333483/1‑90 (MQ=255) gtgtGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGGTTAGCCCCCcact > 1:9117/1‑90 (MQ=255) tgtgGCGCTTGCCGCCCAGGCTATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATTGGAAAAATGAGGTTAGCCCCCcactt > 1:249361/1‑90 (MQ=255) tgtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGAGTTTTGCCCCCcactt > 2:418085/1‑90 (MQ=255) tgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCtacttac < 1:354283/90‑1 (MQ=255) gcATTCAGGCGGGTCCGGCCCAGAGGATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGct < 2:367930/89‑1 (MQ=255) ccATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGGTTAGCCCCCCACTTACTCGATGCAAAAACACGct > 1:163023/1‑90 (MQ=255) cAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCtt < 1:418085/90‑1 (MQ=255) agagCATTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACg > 1:6017/1‑90 (MQ=255) gCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc < 1:248634/90‑1 (MQ=255) gCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTAt < 2:146322/90‑1 (MQ=255) gCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTAt < 1:243606/90‑1 (MQ=255) gCGGGGGGGATGGGAAATATGAGTTTAGCCCCCCACTTACTCGATGCAAAAACACGCTCTGGTTTTCGTCTTGGGGACGGACAGGgtttt > 1:384694/1‑88 (MQ=255) ggggggGATGGAAAAAATGAGTTTAGCCCCCCACTTACTCGATGCAAAAACACGCTCTGGGTATTGTCTTGGAGACGGACAGGGTTATGa > 2:147445/1‑90 (MQ=255) gTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTaa > 1:24969/1‑90 (MQ=255) | TGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAA > NZ_CP009273/2319849‑2320017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |