Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,380,3410GA53.8% 1.4 / 15.4 13intergenic (+31/‑72)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
Reads supporting (aligned to +/- strand):  ref base G (0/6);  new base A (7/0);  total (7/6)
Fisher's exact test for biased strand distribution p-value = 5.83e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAAT  >  NZ_CP009273/2380272‑2380420
                                                                     |                                                                               
gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAAAAAAGCACaatttttttt                                                             >  2:223342/1‑90 (MQ=255)
        tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc                                                     <  1:400788/90‑1 (MQ=255)
                 tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAGAACATTTTTTTTTTCCTCCTCTTTTTTTAg                                            >  2:255526/1‑90 (MQ=255)
                   tGTTATACAACAGTAAATAATAAAAATTTCCCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTTCTCCTCTGTTTTTAGAt                                          >  2:258284/1‑90 (MQ=255)
                              aGTAAATAATAAAAATTTCTCGAGGCGGGTGCACAAAAAAAGACAAGGCCAATTTTTTTTTTCTCATCTCTTTTTAGATGCGAAAAATgg                               >  1:584449/1‑89 (MQ=255)
                                      ataaAAATTTCTCGAGGCGGGTCCCGAAAAAACCACATGCACATTTTATTTTTCCTCCTCTTTTTTTAGATGGGAAAAAAGAAAATTAAc                       >  1:607298/1‑90 (MQ=255)
                                      ataaAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAAAAAGAAAATCaaa                       >  1:503138/1‑89 (MQ=255)
                                              ttCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTTCTCATCTTTTTTTAAATGAGAAAAAAGAAAATTAAAAGAGGaaa               >  2:340451/1‑90 (MQ=255)
                                                  cGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaata           <  2:17875/90‑1 (MQ=255)
                                                     ggCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaataaat        <  1:223342/90‑1 (MQ=255)
                                                        gggTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATggg     <  1:255526/90‑1 (MQ=255)
                                                         ggTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGa    <  1:670217/90‑1 (MQ=255)
                                                           tCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAAt  <  1:340451/90‑1 (MQ=255)
                                                                     |                                                                               
GTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAAT  >  NZ_CP009273/2380272‑2380420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑