| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,126,669 | 0 | A | C | 52.9% | 6.5 / 19.1 | 17 | D216E (GAT→GAG) | yghS | dTMP kinase |
| Reads supporting (aligned to +/- strand): ref base A (2/6); new base C (9/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCA‑GTTGCGGCGTAACGGCGATTTTTTCCTGTAACGC > NZ_CP009273/3126582‑3126737 | atcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGGGTCGCCCCTc > 1:277423/1‑90 (MQ=255) tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGTGTCGCCCATcc < 2:125618/90‑1 (MQ=255) tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGTGTCGCCCATcc < 1:684912/90‑1 (MQ=255) cTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGGGGCGCCCCTcccggt > 1:474965/1‑86 (MQ=255) aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGGGGCGCCCCTCCCGTTCa > 2:30072/1‑90 (MQ=255) gTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGGGGGGCCCCTCCCGTTCAAGGGTCtttt > 2:127098/1‑89 (MQ=255) gATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGTGTCGCCCATCCAGTTCAAGGATCTTTgcg < 2:641936/90‑1 (MQ=255) ggAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGGGGCGCCCCTCCAGGTCAAGGATCTTTTCGCCCtt > 1:368527/1‑90 (MQ=255) aaaGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGTGTCGCCCATcc < 2:617227/65‑1 (MQ=255) aaaGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGGGTCGCCCATcc > 1:617227/1‑65 (MQ=255) gcgcGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGGGGGGCCCCTCCCGTTTAAGGGGCTTTTCGCCCTTGAATg > 2:107026/1‑90 (MQ=255) cggcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGGGGCCCCCCCAG‑TCAAGGGTCTTTTTGGCCTTGAATGttg > 2:419375/1‑88 (MQ=255) gcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGGGTCGCCCATCCCGTTCAAGGGTCTTTTCGCCCTTGAATGTCA‑Gt > 1:252410/1‑90 (MQ=255) aaTTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGGGGCGCCCCTCCCGTTCAAGGATCTTTTTGCCCTTTTATGTCA‑GTTgcgg > 2:144735/1‑90 (MQ=255) gCTTGCAGAATTTCATCGGC‑GGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCA‑GTTGCGGCGTAACGGCGAttttt < 2:368527/90‑1 (MQ=255) tGCAGAATTTCATCGGC‑GGGGGGTCGGCCCTCCCGGTCAAAGATCTTTTTGCCATTTGATGTCAGGTGGGGGGGAAACGGGGTTTTTTTc > 2:444834/1‑90 (MQ=255) ttCATCGGC‑GGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCA‑GTTGCGGCGTAACGGCGATTTTTTCCTGTAACGc < 2:574629/90‑1 (MQ=255) | ATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGC‑GGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCA‑GTTGCGGCGTAACGGCGATTTTTTCCTGTAACGC > NZ_CP009273/3126582‑3126737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |