| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,738,402 | 0 | A | C | 52.9% | 4.3 / 13.6 | 18 | D80A (GAC→GCC) | yiaN | 2,3‑diketo‑L‑gulonate transporter large permease YiaN |
| Reads supporting (aligned to +/- strand): ref base A (4/4); new base C (0/9); total (5/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.94e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGC > NZ_CP009273/3738313‑3738481 | aGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGa < 1:620012/90‑1 (MQ=255) ccTGCTGGCGATTCCGTTTTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACt < 1:684344/90‑1 (MQ=255) tgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACtggtgg < 1:435025/90‑1 (MQ=255) gggCGATTCCGTTTTTTGTGGGGGGGGGTAAAATCATGAAGGGGGGGGGTTTGACAAAACGCATTGTTGCCCTGCCGATGAAACtggtgg < 2:636155/89‑1 (MQ=255) gggCAATTCCGTTTTTTGTGCTGGGGGGTAAAATCATGATTGGGGGCGGTTTGTCAAAACGCATTGTTGCCCTGCCGATGAAACtggtgg < 2:99543/89‑1 (MQ=255) tttCCTTTTTTTGTGCGGGGGGGTAAAATCATGAAGGGGGGGGGTTTGTAAAAACGCATTGTTGCCCTGCCGATGAAACTGGTGGGGCAt < 2:279889/89‑1 (MQ=255) ttCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATa > 1:279889/1‑90 (MQ=255) ccGTTCTTTGGGCTGGGGGGGAAAACCAGCAAGGGGGGCGGTTGGGGAAAACGGGTTGTTGCCCTGCCGATGAAACTGGTGGGGCATaaa < 1:425781/90‑1 (MQ=255) ttttGTGCTGGGGGGTGAAATCATGAATGGGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAgg < 1:514945/89‑1 (MQ=255) ttttGTGCTGGGGGGTAAAATCATGAAGGCGGGGGGTTTGTAAAAACGCTTTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAgg < 2:464607/89‑1 (MQ=255) tGGCGGGGGGAATCATGAAAGCGGGGGGCGTTTCAAAACGCATTGTTGTCCCGCCCGTGAAAATGGGGGGGGATAAAACAGGCggggcgg > 2:669035/1‑86 (MQ=255) tGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCgggc < 1:359174/72‑1 (MQ=255) tGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCgggc > 2:359174/1‑72 (MQ=255) tGCGGGCGGTTTGTAAAACCGCTTTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGc < 2:6072/90‑1 (MQ=255) gggggggggTTTGTAAAACCGCTTTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGc < 2:9568/87‑1 (MQ=255) gggggcggCTTGTCAAAACGCTTTTTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCa < 1:515172/88‑1 (MQ=255) cgggcggCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAg > 1:572643/1‑90 (MQ=255) aCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAg > 2:364969/1‑90 (MQ=255) cgaaTTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGc < 1:160893/87‑1 (MQ=255) | AGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGC > NZ_CP009273/3738313‑3738481 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |