Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,872,3060TG54.5% 8.9 / 13.0 11T392P (ACC→CCC) gyrBDNA topoisomerase (ATP‑hydrolyzing) subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (6/0);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGG  >  NZ_CP009273/3872222‑3872383
                                                                                    |                                                                             
gTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCAttt                                                                          >  1:308658/1‑90 (MQ=255)
   cGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGGATTTTAc                                                                       >  2:391888/1‑90 (MQ=255)
      aaaGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCAcgcg                                                                    <  2:642249/90‑1 (MQ=255)
        aGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCCTTTTTcgcgcg                                                                  >  1:287566/1‑90 (MQ=255)
          cgcCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCCTTTTTcgcccgcg                                                                >  1:263104/1‑90 (MQ=255)
                     gTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCtt                                                     <  2:581072/90‑1 (MQ=255)
                       tCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTca                                                   <  1:435647/90‑1 (MQ=255)
                              aGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTTACCCGCGCGGCGCGCCGCCTcaccgggac                                            >  1:195203/1‑90 (MQ=255)
                                      aGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGgcagca                                    <  1:391888/90‑1 (MQ=255)
                                                                     cACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGt     <  1:163820/90‑1 (MQ=255)
                                                                       ccTTTACGGCGGGGCATTTCCCCCGCGCGGCGCGCCGCCTCACGGGCACGGGCGGCATCGATAATTTTTGCAACCCCCAATTT‑GCGTCgg  >  1:158133/1‑90 (MQ=255)
                                                                                    |                                                                             
GTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGG  >  NZ_CP009273/3872222‑3872383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑