Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,964,4520TG56.0% 24.5 / 32.4 25G320G (GGT→GGG) wecBUDP‑N‑acetylglucosamine 2‑epimerase (non‑hydrolyzing)
Reads supporting (aligned to +/- strand):  ref base T (1/10);  new base G (14/0);  total (15/10)
Fisher's exact test for biased strand distribution p-value = 3.37e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-05
Rejected as consensus: Frequency below/above cutoff threshold.

ATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCA  >  NZ_CP009273/3964369‑3964532
                                                                                   |                                                                                
aTTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTg                                                                            <  2:341379/90‑1 (MQ=255)
    aGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTc                                                                        <  1:3472/90‑1 (MQ=255)
    aGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTc                                                                        <  2:265098/90‑1 (MQ=255)
        agaagCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGGCGGGGGGGCTGGt                                                                    >  1:389823/1‑90 (MQ=255)
        agaagCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGGGGCTggg                                                                    >  1:514301/1‑89 (MQ=255)
          aagCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGGGGCTggggg                                                                  >  2:194250/1‑87 (MQ=255)
            gcgcCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGc                                                                <  2:11758/90‑1 (MQ=255)
                   cGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATa                                                         <  2:117247/90‑1 (MQ=255)
                        gggAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAagcag                                                    <  2:36427/90‑1 (MQ=255)
                        gggAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAagcag                                                    <  2:626733/90‑1 (MQ=255)
                                 gtgCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGGGTCTGGGTGGGACGGATAAGCAGCGGAATGTc                                           >  1:462594/1‑90 (MQ=255)
                                   gCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGa                                         <  1:152907/90‑1 (MQ=255)
                                           tGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGGGGCTGGTAGGGACGGGTAAACAGCGGAATGTCGGgggaggga                                 >  2:190989/1‑90 (MQ=255)
                                                gATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGGGTCTGGGAGGGACGGGTAAGCAGCGAAATGTCGGGGGAGGGAcgcgg                            >  2:168002/1‑89 (MQ=255)
                                                      aCTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGGGCGGCTGGTAGGCACGGATAAACAGCGAATTGTCGAGGAAGTGACGCGGCttttt                      >  1:197653/1‑89 (MQ=255)
                                                         gAGCGTCCGGAAGCGGTGACGGCGGGGCCGGGGCGTCTGGGAGGCGCGGATAAACAGCGGAGTGTGGGGGGAGGGACGCGTCTTTTtaaa                   >  1:680367/1‑90 (MQ=255)
                                                         gAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGGGTCTGGGAGGCACGGATAAACAGCGAAATGTTGAGGGGGGGACGCGGCTTTTtaaa                   >  1:443836/1‑90 (MQ=255)
                                                         gAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGGGGCTGGTTGGGACGGATAAAAAACGAATTGTCGAGGGAGGGGCGCGGCTTTTtaaa                   >  1:433968/1‑90 (MQ=255)
                                                           gCGTCCGGAAGCGGTGACGGCGGGGACGGGGCGTCTGGTAGGGACGGGGAAGAAGCGAATTGTCGGGGGAGGGAGGGGGCTTTTTAAAGa                 >  2:240520/1‑90 (MQ=255)
                                                           gCGTCCGGAAGCGGTGACGGCGGGGACGGGGCGTCTGGTAGGGAAGGATAAACAGGGGAATGTCGGGGGAGGGACGCGGCTTTTTAAAGa                 >  2:150909/1‑90 (MQ=255)
                                                             gTCCGGAAGCGGTGACGGCGGGGACGGTGCGGCGCGGGGGGACGGAGAAAGAGGGGATTGTTGGGGGAGGGGCGCGGCTTTTtaaaaaag               >  2:297597/1‑86 (MQ=255)
                                                             gTCCGGAAGCGGTGACGGCGGGGACGGGGCGTCTGGGAGGGACGGGTAAACAGCGGAATGTTGGGGGAGGGGCGCGGCTTTTtaaaaaag               >  2:89992/1‑86 (MQ=255)
                                                             gTCCGGAAGCGGTGACGGCGGGGACGGGGCGGCTGGGAGGCACGGGTAAGCAGCGAAATTTGGGGGGAGGGGCGCGGCTTTTtaaaaaag               >  1:357218/1‑86 (MQ=255)
                                                                    aGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGa        <  2:62848/90‑1 (MQ=255)
                                                                          gACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCa  <  2:253708/90‑1 (MQ=255)
                                                                                   |                                                                                
ATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCA  >  NZ_CP009273/3964369‑3964532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑