| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,086,494 | 0 | T | G | 64.7% | 0.9 / 12.5 | 17 | Y102S (TAC→TCC) | rhaM | L‑rhamnose mutarotase |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (11/0); total (13/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAA > NZ_CP009273/4086421‑4086583 | gTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAActtttttccg > 2:573718/1‑86 (MQ=255)gTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAg > 2:548550/1‑90 (MQ=255) tttAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGc > 1:490541/1‑90 (MQ=255) tttAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAAAATCTTTCAGc > 2:454619/1‑90 (MQ=255) tttCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACCTTTTTCCGCTCGCCAc > 2:152825/1‑90 (MQ=255) aaaTTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCt < 1:481990/90‑1 (MQ=255) tgCCGGAGGCATCTCTGGCACATTGGGCAATTCCGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCCCCGGGCTGTTATCCGGGTTAGc < 2:577484/90‑1 (MQ=255) ggAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTTAAACACCTCTTTCCGGCCGCGACCCCCCCGGCCGTTATACGGGGTAGCGGGc > 1:577484/1‑90 (MQ=255) aGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAACAATTCTTTCAGCTCGCCCCTCACCCGGCCGTTTTTCGGGGTAGCGGGCAt > 2:231087/1‑90 (MQ=255) aGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACCTCTTTCCGCTCGCCACTCCCCCGGCTGGTTTCCCGGGTAGCGGGGAt > 2:167904/1‑90 (MQ=255) gctctGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACAt < 1:288032/89‑1 (MQ=255) ctctGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATc < 1:454619/90‑1 (MQ=255) acaTTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGGCata > 1:102765/1‑90 (MQ=255) ttGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCGCTACTCACCCGGCTGTTTTCCGGGGTAGCGGGGATAAAAACGGGCatatac > 2:288032/1‑89 (MQ=255) gggCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCCCCACTCCCCCGGCTGTTTTTCGGGGTAGCGGGCATAACATCGGGCATATAttt > 2:608976/1‑90 (MQ=255) ttACGGCAGGGAAAAAACTTCTTTCCGCTCGCCACTCACCCGGCTGTTTTCCGGGGTAGCGGGCATAACATCGGGCATATATTTtcacca > 2:355569/1‑90 (MQ=255) ggCAGGGAAAAAACTTCTTTCAGCTCGCTACTCACCCGGCTGTTTTTCGGGGTAGCGGGCATAACATCGGGCATATATTTCCCCCAACGt > 2:620222/1‑90 (MQ=255) tAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCaa < 2:3628/90‑1 (MQ=255) tAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCaa < 1:675005/90‑1 (MQ=255) tAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCaa < 1:573718/90‑1 (MQ=255) | GTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAA > NZ_CP009273/4086421‑4086583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |