| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,086,507 | 0 | G | T | 57.1% | 17.2 / 31.3 | 21 | Q98K (CAA→AAA) | rhaM | L‑rhamnose mutarotase |
| Reads supporting (aligned to +/- strand): ref base G (1/8); new base T (12/0); total (13/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.42e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.95e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGC > NZ_CP009273/4086421‑4086593 | gTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAg > 2:548550/1‑90 (MQ=255) tttAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGc > 1:490541/1‑90 (MQ=255) tttAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAAAATCTTTCAGc > 2:454619/1‑90 (MQ=255) tttCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACCTTTTTCCGCTCGCCAc > 2:152825/1‑90 (MQ=255) aaaTTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCt < 1:481990/90‑1 (MQ=255) tgCCGGAGGCATCTCTGGCACATTGGGCAATTCCGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCCCCGGGCTGTTATCCGGGTTAGc < 2:577484/90‑1 (MQ=255) ggAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTTAAACACCTCTTTCCGGCCGCGACCCCCCCGGCCGTTATACGGGGTAGCGGGc > 1:577484/1‑90 (MQ=255) aGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAACAATTCTTTCAGCTCGCCCCTCACCCGGCCGTTTTTCGGGGTAGCGGGCAt > 2:231087/1‑90 (MQ=255) aGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACCTCTTTCCGCTCGCCACTCCCCCGGCTGGTTTCCCGGGTAGCGGGGAt > 2:167904/1‑90 (MQ=255) gctctGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACAt < 1:288032/89‑1 (MQ=255) ctctGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATc < 1:454619/90‑1 (MQ=255) acaTTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGGCata > 1:102765/1‑90 (MQ=255) ttGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCGCTACTCACCCGGCTGTTTTCCGGGGTAGCGGGGATAAAAACGGGCatatac > 2:288032/1‑89 (MQ=255) gggCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCCCCACTCCCCCGGCTGTTTTTCGGGGTAGCGGGCATAACATCGGGCATATAttt > 2:608976/1‑90 (MQ=255) ttACGGCAGGGAAAAAACTTCTTTCCGCTCGCCACTCACCCGGCTGTTTTCCGGGGTAGCGGGCATAACATCGGGCATATATTTtcacca > 2:355569/1‑90 (MQ=255) ggCAGGGAAAAAACTTCTTTCAGCTCGCTACTCACCCGGCTGTTTTTCGGGGTAGCGGGCATAACATCGGGCATATATTTCCCCCAACGt > 2:620222/1‑90 (MQ=255) ggaaaaaaCATCTTTCAGCTCGCTACTCACCCCGCCGTTATCCCGGGTAGGGGGCATAAAAACGGTCATATATTTCCACCAACCTTTGCa > 2:516605/3‑90 (MQ=255) tAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCaa < 1:675005/90‑1 (MQ=255) tAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCaa < 1:573718/90‑1 (MQ=255) tAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCaa < 2:3628/90‑1 (MQ=255) cttGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGc < 1:548550/90‑1 (MQ=255) | GTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGC > NZ_CP009273/4086421‑4086593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |