| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 115,871 | 0 | T | G | 62.5% | 7.2 / 14.5 | 16 | V35G (GTG→GGG) | ampE | beta‑lactamase regulator AmpE |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (10/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGG > NZ_CP009273/115786‑115955 | ttACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGGGaaa > 1:40379/1‑90 (MQ=255)ttACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGGGaaa > 2:65170/1‑90 (MQ=255) tgtTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGGGAAAAAttttt > 1:320242/1‑90 (MQ=255) ttAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGGGAAACAttttttt > 1:55768/1‑88 (MQ=255) tAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAAAttttttt > 2:90649/1‑88 (MQ=255) tCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGTGAAACATTTTTCTCTCGGgc < 1:244557/90‑1 (MQ=255) gAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGTGAAACATTTTTCTCTCGGgcgc < 1:301801/90‑1 (MQ=255) tGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGGGGAAAATTTTTTTCTCTGGGGCGCGTTTGGGATGACCCTTTTTGCGATg > 2:240079/1‑90 (MQ=255) gCTTGATCTTCGTCTTGAAGCGTTCTTTCGGC‑GGGGGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACTATTATTGCGATGGGCGt > 1:41417/1‑90 (MQ=255) gCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGGGAAAAAATTTTTTCTCGGGGGGCCGGTAGGGGTGACCATTTTTTTGAGGGGCgg > 2:376283/1‑89 (MQ=255) tcGTCTTGAAGCGTTCTTTCGGC‑GGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACtttttt < 1:115194/90‑1 (MQ=255) cGTCTTGAAGCGTTCTTTCGGC‑GGGGGAAAAATTTTTTTCTCGGGCGCACGTTAGGCATGACCATTTTTTCGGTGGGGGGGAAttttttt > 1:316202/1‑89 (MQ=255) gTCTTGAAGCGTTCTTTCGGC‑GGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTAc < 1:265097/90‑1 (MQ=255) gTTCTTTCGGC‑GGGGGAAACATTTTTTTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACgcgc > 2:108314/1‑90 (MQ=255) cggc‑ggGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAg < 2:378803/90‑1 (MQ=255) gc‑ggGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAggg < 2:314839/90‑1 (MQ=255) | TTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGG > NZ_CP009273/115786‑115955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |