| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 296,422 | 0 | G | C | 55.6% | 8.0 / 24.8 | 18 | P75R (CCG→CGG) | paoC | aldehyde oxidoreductase molybdenum‑binding subunit PaoC |
| Reads supporting (aligned to +/- strand): ref base G (1/7); new base C (10/0); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGG > NZ_CP009273/296337‑296503 | ggggCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGGATGACAGCCCGCACGCCCCgcgc > 2:377838/1‑90 (MQ=255) cgccTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAAAGACAACCCGTACGCCCCGCGCttttt > 1:58240/1‑90 (MQ=255) cgccTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAATGACAGGCAGTACGCCCCGGGCttttt > 1:97818/1‑90 (MQ=255) ccTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAAAAACAGCCAATACGCCCCGCCCctttttg > 1:301771/1‑88 (MQ=255) tAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTgcgc < 1:48708/90‑1 (MQ=255) tGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACCCCCCGCGCTTTTTTCGCGGCGTCCg > 1:141445/1‑90 (MQ=255) gtTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCCGCGCTTTTTTCGCGGCGTCCGTATCAAg > 1:80971/1‑90 (MQ=255) tttGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCg < 1:287825/90‑1 (MQ=255) gTGCCCCGGCGTTACTGGCGGTAATTACAACCCCTACGCCCCGCGCCTTTTGCGCGGCGTCCGGATCAAGGGGGGGGAGGCGGCCttttg > 1:149055/1‑88 (MQ=255) gTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCCGCGCTTTTTGCGCGGCGTCCGTATCAAGGGGGGGGGGGCGGCCTTTgg > 1:387523/1‑90 (MQ=255) ccccGGCGTTACTGGCGGTAATGACAACCAGGACGCCCCGCGCTTTTTTCGCGGCGGCCGGATCAAGGGCGGGGAGGCGGCCTTTGGCaa > 2:327495/1‑90 (MQ=255) gTTACTGGCGGTAATGACAGCCAGTACGCCCCGCGCCTTTTTCGCGGCGGCCGGATCAAGGGGGGGGGGGGGGCCCTTGGCCATGGCggg > 2:368493/1‑89 (MQ=255) cTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACcg < 2:97818/90‑1 (MQ=255) ggCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACcgac < 1:378118/90‑1 (MQ=255) ggCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACcgac < 1:259204/90‑1 (MQ=255) ggggTAAGGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACcgacg < 2:220317/88‑1 (MQ=255) tAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGatat < 2:387866/90‑1 (MQ=255) gTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAggcgg > 2:93338/1‑90 (MQ=255) | GGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGG > NZ_CP009273/296337‑296503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |