| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 702,597 | 0 | T | G | 54.5% | 7.0 / 10.4 | 11 | V350G (GTG→GGG) | glnS | glutamine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base G (6/0); total (8/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAACACCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGG > NZ_CP009273/702508‑702675 | caacaCCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGt < 2:76229/90‑1 (MQ=255) acCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGaaa < 1:233976/90‑1 (MQ=255) aTTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAAcag > 2:183012/1‑88 (MQ=255) aTTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGGGAAACTg > 1:93813/1‑90 (MQ=255) ggCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGGGAAACTGGTTATCGa > 1:124353/1‑90 (MQ=255) ggCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGGTTTCGATCCCGGGGAAAAGGTTATTGa > 2:41728/1‑90 (MQ=255) cGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGaaa < 2:128144/90‑1 (MQ=255) gTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGaaaa > 1:402078/1‑90 (MQ=255) tcAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCa < 2:402078/90‑1 (MQ=255) aaaaTGCGCCGCGCGCAATGGCGGTTATCGATCCGGGGGAAATGGTTATCGAAAAATATCAGGGGGAAGGGGAAAAGGGTAACAAGCCGa > 1:356204/1‑90 (MQ=255) aaaaTGCGCCGCGCGCAATGGCGGTTATCGATCCCGGGAAAATGGTTATCGAAAAATATCAGGGCGAAGGGGAAAAGGTTACCATTCCGa > 1:196916/1‑90 (MQ=255) tATTGATCCCGGGGAACAGGGTTTTGAAAAAAATCAAGGGGGAGGGGGAAAGGGTACCATGCCGAACCAACCCCACAAAACGGAAAAggg > 2:385857/1‑90 (MQ=255) | CAACACCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGG > NZ_CP009273/702508‑702675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |