| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,329,948 | 0 | T | G | 53.8% | 9.0 / 10.3 | 13 | intergenic (+24/‑140) | BW25113_RS25560/acnA | hypothetical protein/aconitate hydratase AcnA |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base G (7/0); total (10/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGT > NZ_CP009273/1329864‑1330025 | gggCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCa > 2:320181/1‑90 (MQ=255) tCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTTCCACAAg > 2:114301/1‑90 (MQ=255) aTGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGtt > 1:25650/1‑90 (MQ=255) cttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTctcc < 2:302816/90‑1 (MQ=255) aTTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTTCCACAAGGTTTTTCCTCTTTTTTCAATTTGGGGTGTTATc > 2:271943/1‑90 (MQ=255) cACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTTTCAATTTGGGTTGTTATCAAATCg > 1:48185/1‑90 (MQ=255) cACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTAt > 1:72572/1‑67 (MQ=255) cACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTAt < 2:72572/67‑1 (MQ=255) gATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAc < 2:287628/90‑1 (MQ=255) tGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTTTCCTCTTTTTTCAATTTGGGGTGTTTTCAAATCGTTACGCGATg > 2:150906/1‑90 (MQ=255) ccGCTGCGGCGGGGGTGTCATTTTCTTTGCCACAAGGGTTCTCCTCTTTTTTCAATTTGGGTTGTTATCAAATCGTTACGCGATGttttc > 1:287628/1‑88 (MQ=255) cGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGGTTCTCCTCTTTTATCAATTTGGGGTGTTTTCAAATCGTTACGCGATGTTtgtg > 1:31072/1‑90 (MQ=255) gCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTgtgt > 1:17969/1‑90 (MQ=255) | GGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGT > NZ_CP009273/1329864‑1330025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |