| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,570,334 | 0 | C | A | 54.5% | 8.1 / 16.5 | 11 | D515Y (GAT→TAT) | yddB | TonB‑dependent receptor plug domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (4/1); new base A (0/6); total (4/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAG > NZ_CP009273/1570247‑1570419 | gCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCaa > 1:207306/1‑90 (MQ=255) ttttCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGaa < 2:269437/90‑1 (MQ=255) gATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGAAATCCCATATCAAGAATATTCCCGCCAt > 2:405409/1‑90 (MQ=255) gcgTTAGATTTTTATTCCCTGATCCCGATCCCGCCCAGCTATTGCGGATATCACGTAACCCCATATAAAAAATATTCCCGCCATAGTAAc < 1:26004/90‑1 (MQ=255) gcgTCAGATTTTTTTTCCCTGATCCCGTTCCCGCCCAGCTTTTGCGGATATCACGTAACCCCATATAAAGAATATTCCCGCCATAGTAAc < 1:9749/90‑1 (MQ=255) tttATTCCCTAATACCTTTCCCCCCCAGTTTTGGGGAATATCACAAAACCCCATAAAAAAAATTTTCCCGCCATAGTAACGGTTATAAcc < 2:94577/90‑1 (MQ=255) tttATTACCTGATCCCGTTCCCGCCCAGTTATTGGGGATATCACGTAACCCCATATAAAAAATATTCCCGCCATAGTAACGGTTATAAcc < 1:155069/90‑1 (MQ=255) tcccTGTTCCCGTTCCCCTCCAGTTTTTGGGGATATCAGGTAACCCAATAAAAAAATTTTCCCCGCCATAGTAACGGTTATAACctgctg < 2:89033/88‑1 (MQ=255) gTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGa > 1:273317/1‑90 (MQ=255) tGCGGATATCACGTAACCCCAAAAAAAAAATTTCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCaaaaa < 1:319563/90‑1 (MQ=255) atatCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAg > 1:169747/1‑90 (MQ=255) | GCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAG > NZ_CP009273/1570247‑1570419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |