| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,216,154 | 0 | T | G | 53.8% | 5.9 / 11.1 | 13 | V164G (GTG→GGG) | dld | D‑lactate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base G (7/0); total (10/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTG > NZ_CP009273/2216070‑2216236 | gcgAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGGATTTTTTACAAATCCGGCGGCTCCCCGGTGCAAc > 2:390112/1‑90 (MQ=255)gcgAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGGATTTTTAACAACTCCCGCGGCTCGCTGGGGCAAc > 1:240134/1‑90 (MQ=255) cgAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACg < 2:170963/90‑1 (MQ=255) cgAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACg < 2:182491/90‑1 (MQ=255) cAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTTTAAAAAATCCGGCGGCTCGCTGGGGGAACGGGGCCCGGCgg > 1:26999/1‑89 (MQ=255) tGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAAAAACTCCGGCGGCCCGCTGGTGCAACGTGGGCCCGCGgata > 1:87532/1‑90 (MQ=255) ggATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGaa > 1:122799/1‑90 (MQ=255) catcGTGTATAGGCGCATCGGTCATCGGCGGTATTTTTCACAACCCCTGCGGCTCTCTGGGGCAACGTGGACCGGCGCGTAACGAAATGt > 2:164725/1‑90 (MQ=255) ataGGCGCATCGGTCATCGGCGGTATTTTTAAAAACTCCCGCGGCTCGCCGGGGGAACGGGGCCCCGCGGATAACGAAAAGTCGTTATTc > 2:391891/1‑90 (MQ=255) ggCGCATCGGTCATCGGCGGTATTTTTAAAAACTCCGGCGGCTCGCTGGGGGAACGTGGCCCCGCGGATACCGAAAAGTCGTTATTcgcg > 2:136978/1‑90 (MQ=255) gcATCGGTCATCGGCGGTATTTTTAACAAATCCCGCGGCTCGCTGGGGCAACGGGGCCCGGCGGATACCGAAAAGTCGGTTTTTGCGCGt > 2:158706/1‑90 (MQ=255) aTCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGGGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGtat > 1:122778/1‑90 (MQ=255) tCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTg < 2:122799/90‑1 (MQ=255) | GCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTG > NZ_CP009273/2216070‑2216236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |