| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,109 | 0 | C | A | 61.5% | 4.6 / 17.1 | 13 | L419L (CTC→CTA) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (3/2); new base A (0/8); total (3/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTA > NZ_CP009273/3214024‑3214191 | tGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATa < 1:154159/90‑1 (MQ=255) aTCGGTTATATTTTGGCCAGCGAGATTTCCCCCCAGCGCGTACTGGGGGCCGAAACCCTAAAAACCCCCAAAACGACCCGCATTAAACCg < 2:130611/90‑1 (MQ=255) tttGCCGGGGAGATGTCCCGCCAGCGCGTACTGGTGGCGGGAACGCTAAAAAACGCAAAAACGACCCGCATTGAACCGCcactgacactg < 1:172123/90‑1 (MQ=255) tttCCCAGGGAGTTGTCCCCCCGGCGCGTACGGGTGGCCGAACCGCTAAATAACGCAAAAACGACCCGCATTGAACCGCcactgacactg < 1:184071/90‑1 (MQ=255) tttCCCAGCGAGATGTCCCGCCAGCGCTTACTGGTGGCCGGACCGCTAAATAACCCCAAACCGACCCGCATTGAACCGCcactgacactg < 1:254347/90‑1 (MQ=255) tGCCAGGGAGATGTCCCGCCAGCGCGTACGGGGGGCCGGAACGCTAAAAAACGCAAAAACGACCCGCATTAAACCGCCactgacactgac < 1:169505/90‑1 (MQ=255) agaTGTCCCCCCGGGGCGTGCGCGTGGCGGGAACGATAAAAAACGCAAAAACTACCCTTATTGAACCGCCACTGACACTGACCATTGAAc < 1:396718/90‑1 (MQ=255) ttcccgccgGCGCGTTGGGGGGGCGGGAACGCTAAAAAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAgtg < 1:394868/87‑1 (MQ=255) gcgcGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGt > 1:272345/1‑90 (MQ=255) tACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCa < 1:67843/42‑1 (MQ=255) tACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCa > 2:67843/1‑42 (MQ=255) gggCCGGACCGCTAAAAACCCCAAAAACGACCCGCATTAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcgg < 1:283334/89‑1 (MQ=255) gAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTa > 1:589/1‑90 (MQ=255) | TGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTA > NZ_CP009273/3214024‑3214191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |