| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,348,816 | 0 | A | C | 57.9% | 15.7 / 23.2 | 19 | T245P (ACC→CCC) | gltB | glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base A (7/1); new base C (0/11); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTC > NZ_CP009273/3348750‑3348902 | gTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcgt > 1:218455/1‑90 (MQ=255) cgcgcgGGCCGCGGGCGAACCCGTCCCGCTATCGGGCGCAAAACGGTGAAATCAACCCCACCCCCGGTAACCGCCAATGGgcgcgtgcgc < 1:65545/90‑1 (MQ=255) gcgcTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcgtgcgcg > 2:289992/1‑90 (MQ=255) gcgcTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcgtgcgcg > 2:65545/1‑90 (MQ=255) cgcgggcGCACCCGTCCCGCTATCTGGCGAATAACGGTGAAACCACCCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTtata < 1:118062/90‑1 (MQ=255) ccacccGTCCCGCTTTCGGGCGAATAAGGGTGAAATCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCa < 1:272308/86‑1 (MQ=255) cGTCCCGCTATCGGGCGCATAAGGGTAAAACCAACCCCACCCCGGGTACCCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGacac < 2:318087/90‑1 (MQ=255) ggttATCGGGCGCATACCGGTAAAATCACCCCCACCCCGGGTACCCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCtt < 1:326978/87‑1 (MQ=255) cGCTTTCTGGCGAAAACGGGAAAAACCAACCCCACCCCCGGAACCCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCtt < 2:101638/90‑1 (MQ=255) cGCTATCTGGCGCATAAGGGTAAAATCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCtt < 1:159480/90‑1 (MQ=255) cGCTATCGGGCGAAAAACGGTAAAACCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCtt < 1:289992/90‑1 (MQ=255) aTCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGac < 1:24919/79‑1 (MQ=255) aTCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGac > 2:24919/1‑79 (MQ=255) tcgggCGAAAAAGGGAGAAACCACCCCCACCCCCGGTACCCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATccc < 2:15825/87‑1 (MQ=255) tCTGGCGCAAAAGGGTAAAATCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATccc < 1:247614/90‑1 (MQ=255) gcgcATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGAc > 1:5173/1‑90 (MQ=255) aaCGGTAAAATCAACCCCACCCCCGGTACCCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCac < 2:99104/90‑1 (MQ=255) aaCACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCAcct > 1:197789/1‑89 (MQ=255) aTCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTc > 1:14145/1‑90 (MQ=255) | GTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTC > NZ_CP009273/3348750‑3348902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |