| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,401,190 | 0 | C | G | 54.5% | 6.1 / 12.9 | 12 | G75G (GGC→GGG) | panF | sodium/pantothenate symporter |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base G (6/0); total (9/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCT‑GGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGC > NZ_CP009273/3401109‑3401274 | ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCCGGGGCTGCTTTTAAAAACGGGGT‑GGGGTGGGGAtt > 1:357474/1‑90 (MQ=255)ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTTTAAATACGGGGT‑GGGGTGGGGAtt > 1:76002/1‑90 (MQ=255) cACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTTTAAAAACGGGGT‑GGGGTGGGGATTGCTGGGggtgat > 1:261707/1‑90 (MQ=255) gACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCCGGAGCGGCTTTTTAAAACGGGGT‑GGGGGGGGGATTGTTGGCGGTGATTTAGCt > 1:289123/1‑90 (MQ=255) gACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCT‑GGGCTGGGTATTGCTGGCGATGATTCAGCt < 1:230275/90‑1 (MQ=255) cAGTGCCAGTTCGTTTATCGGCGGGGCCGGAGCCGCGTTTAAAAAAGGGGTCGGGTTGGGTTTTG‑TGGGGGTGATTTAGCTTTCTGCact > 2:258512/1‑88 (MQ=255) aGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCT‑GGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTc < 2:187681/90‑1 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGGGGTGCTTATAAAAAAGGGCT‑GGGGTGGGGGTTTGTGGGGGTGATTTTGCTTCCTGCAGTCt > 2:128586/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTATAAATACGGGCT‑GGGGTGGGGATTGGTGGCGGTGATTCAGCTTCCTGCAGTCt > 1:48204/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGAGCCGCTTATAAATAAGGGCT‑GGGCTGGGGATTGCTGGCGATGCTTCAGCTTCCTGCAGTCt > 1:29076/1‑90 (MQ=255) cGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCT‑GGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCAc < 1:32146/90‑1 (MQ=255) aCGGGCT‑GGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTg > 2:196606/1‑90 (MQ=255) ct‑gggctgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcgcgc > 2:354962/1‑90 (MQ=255) | GGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCT‑GGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGC > NZ_CP009273/3401109‑3401274 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |