| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,571,661 | 0 | A | C | 54.5% | 10.0 / 12.4 | 11 | G142G (GGT→GGG) | gntR | gluconate operon transcriptional repressor GntR |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/6); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCC > NZ_CP009273/3571581‑3571734 | cGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACt > 2:232574/1‑90 (MQ=255) gctgctTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCa > 2:264448/1‑90 (MQ=255) gctTAAAAGTTAAAAAACCCGAGGGCGATACAAGGGCAGGGCGTCTTGTTGTCCATCAGTCCCCCCAGGGAAATCCCCGCCACTTCAatc < 1:332470/90‑1 (MQ=255) ctTAAAATTTACAAAACCCGACGGCAATATAAAGGCAGGGCGCCTTGCTGTCCATCATTCCCCCCACGGAAATCCCCGCCACTTCAatca < 1:46067/90‑1 (MQ=255) acccgccgGCAATATCAAGGCATGGCGACTTGCTGCCCATCATTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGcgcg < 1:108048/88‑1 (MQ=255) ggggCAATATCAGGGCGGGGCGTCTTGCTGTCCATCAGTTCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCgtg < 1:319525/88‑1 (MQ=255) aagggCGTCTTGTTGTCCATCATTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTg < 2:129776/87‑1 (MQ=255) tGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAg > 2:116110/1‑90 (MQ=255) tGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAg > 2:376526/1‑90 (MQ=255) gttttgttgCCCATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATc < 2:38870/87‑1 (MQ=255) gTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTcc > 1:265203/1‑90 (MQ=255) | CGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCC > NZ_CP009273/3571581‑3571734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |