Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,594,1610CT56.5% 3.0 / 26.2 23S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
Reads supporting (aligned to +/- strand):  ref base C (1/9);  new base T (13/0);  total (14/9)
Fisher's exact test for biased strand distribution p-value = 1.22e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.06e-04
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC  >  NZ_CP009273/4594087‑4594238
                                                                           |                                                                             
gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATgag                                                                >  1:203221/1‑90 (MQ=255)
          tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGGgcgc                                                      >  1:326019/1‑90 (MQ=255)
          tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                      <  1:208790/90‑1 (MQ=255)
          tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                      <  2:121042/90‑1 (MQ=255)
           ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATTCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTTAGAAGTGAgcgc                                                      >  2:333313/1‑90 (MQ=255)
           ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGTGAGCGCa                                                     >  1:95772/1‑90 (MQ=255)
           ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTGTGAAGAGAGCGCa                                                     >  2:251441/1‑90 (MQ=255)
                aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCagagag                                                >  1:318006/1‑90 (MQ=255)
                aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCagaggg                                                >  1:201678/1‑88 (MQ=255)
                aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCagagag                                                >  2:180463/1‑90 (MQ=255)
                aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCagagag                                                >  1:263753/1‑90 (MQ=255)
                 gTCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCAGAGAgg                                               >  1:328234/1‑90 (MQ=255)
                  tCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAggg                                              >  2:153200/1‑90 (MQ=255)
                   agggAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa                                             <  1:364133/88‑1 (MQ=255)
                            tttCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc                                    <  2:35930/90‑1 (MQ=255)
                             ttCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGcc                                   <  2:37049/90‑1 (MQ=255)
                              tCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCct                                  <  2:73250/90‑1 (MQ=255)
                                           tCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCATGAGAAGGGAGCGCAGAGGGGGGTTATCTTGCCTGACTGAACTGGCg                     >  1:127793/1‑90 (MQ=255)
                                             gcgGA‑TCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATTTTGCCTGACTGAACTGGCGAt                   >  2:388554/1‑90 (MQ=255)
                                                     tcGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGGTTAAGttt            >  1:391323/1‑90 (MQ=255)
                                                               cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc  <  2:201678/90‑1 (MQ=255)
                                                               cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc  <  1:333313/90‑1 (MQ=255)
                                                               cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc  <  1:251441/90‑1 (MQ=255)
                                                                           |                                                                             
GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGA‑TCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCC  >  NZ_CP009273/4594087‑4594238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑