| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,596,719 | 0 | A | G | 56.2% | 0.4 / 13.0 | 16 | S267P (TCC→CCC) | rsmC | 16S rRNA (guanine(1207)‑N(2))‑methyltransferase RsmC |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base G (9/0); total (12/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACA > NZ_CP009273/4596632‑4596786 | cgcTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGgaga < 1:205056/90‑1 (MQ=255) tGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGGTGGGGGGGATCatatta > 2:3715/1‑88 (MQ=255) gACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGGGATGATCATATCaa > 1:303546/1‑90 (MQ=255) gACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGGTGGGGAAGAACATATCaa > 2:172047/1‑90 (MQ=255) gcaccgcaccGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAAc < 1:237657/90‑1 (MQ=255) ccgcGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGGTGGGGGTTATCATATCAAAACAACCTTTc > 2:187674/1‑90 (MQ=255) gCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGGTGGGGATGATCATATCAAAACGACCCTTCACCTCGGgaa > 2:225079/1‑90 (MQ=255) ccgcATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGc < 1:280183/90‑1 (MQ=255) gggTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACtt < 2:359666/89‑1 (MQ=255) ggTTTGCATCCCATCGTGGAACGGCGGGTTGGGGATGATCATATCAAAACGACCTTTCACCTCGGAAAAAACGTTGCTGGGAAAGACTTc > 1:340879/1‑90 (MQ=255) ggTTTGCATCCCATCGTGGAACGGCGGGTTGGGGATGATCAAATTAAAAAGAACTTTTCCCCCGGGAAAAACGCTGGTCGCCAAAACTTc > 2:282468/1‑90 (MQ=255) ggTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTc > 1:29098/1‑90 (MQ=255) ggTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTc < 2:29098/90‑1 (MQ=255) ggTTTGCATCCCATCGTGGAACGGCGGGGTGGGGATGATCATATCAAAAAGACCTTTTACCCCGGGAAAAACGTTTGTGGCAAAAAATTc > 2:105546/1‑90 (MQ=255) gCATCCCATCGTGGAACGGCGGGGTGGAGGTGGTTATATTAAAACGACCTTTCACCTCGGGAAAGACGTTGCTGGGGAAAAATTCCCCtt > 1:313401/1‑90 (MQ=255) ccATCGTGGAACGGCGGGTTGGAGATGGTCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTTAaca > 1:31266/1‑90 (MQ=255) ccATCGTGGAACGGCGGGGTGGGGATGATCATATCAAAACGACCTTTCCCCTCGGAAAAGACGTTGCTGGCAAAGACATCACCTTCAacc > 1:359666/1‑89 (MQ=255) | CGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACA > NZ_CP009273/4596632‑4596786 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |