Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,910	T→G	W30G (TGG→GGG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,910	0	T	G	100.0%	30.5 / NA	9	W30G (TGG→GGG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (1/8);  total (1/8)

CGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49839‑49996                                                                        |                                                                                       cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCt                                                                      <  2:168955/90‑1 (MQ=255)        aGCGGTAGATCGCGTTATCGGCATGGGAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTtaaataa                                                               <  1:337163/90‑1 (MQ=255)        aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTtaaataa                                                               <  1:296056/90‑1 (MQ=255)        aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTtaaataa                                                               <  1:400834/90‑1 (MQ=255)            gTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAccc                                                           <  2:234825/90‑1 (MQ=255)                         tCGGCATGGAAACCGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgcc                                              <  2:100433/90‑1 (MQ=255)                              aTGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                         <  1:300795/90‑1 (MQ=255)                                                         cctaccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTg              >  1:40156/5‑90 (MQ=255)                                                                     gCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  1:174121/90‑1 (MQ=255)                                                                        |                                                                                       CGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49839‑49996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑