Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,569,3160AC54.5% 7.0 / 12.2 11V369G (GTG→GGG) gntUgluconate transporter
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGC  >  NZ_CP009273/3569246‑3569391
                                                                      |                                                                           
accaGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTaa                                                          >  1:354965/1‑90 (MQ=255)
            aaTCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGt                                              >  2:126610/1‑90 (MQ=255)
                 ccgccgccgcCATTTGCGCACGGGAGTATTTCATTTTTAAAAAACCCGCCATCCCCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAg                                         <  2:354965/86‑1 (MQ=255)
                 ccAGCCCCGCCTTTTGCGCACCGGAGTAGTTCAGTTTTTAAAAAACCGGCATCCCCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAg                                         <  1:314896/89‑1 (MQ=255)
                    gcgcCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAAc                                      >  1:84748/1‑90 (MQ=255)
                        cgccATTTGCCCCCCGGAGTAGTTCAGTTGTTCAATACCCGGCATCCCCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTg                                  <  1:92619/90‑1 (MQ=255)
                          ccttttGCCCCCGGGAGTATTTCATTTGTTAAATAACCGGCATCCCCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGaa                                <  1:140287/87‑1 (MQ=255)
                                    ccgggAGTAGTTCAGTTGTTCAAAACCCGGCATCCCCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCAc                      <  1:44145/87‑1 (MQ=255)
                                             gtTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGc             >  2:253296/1‑90 (MQ=255)
                                                       tCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAg   >  1:92925/1‑90 (MQ=255)
                                                        aaaTACCCGCCACCCCCAGTCCTCCCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGc  <  1:292267/89‑1 (MQ=255)
                                                                      |                                                                           
ACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGC  >  NZ_CP009273/3569246‑3569391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑