Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	481,482	T→G	L89* (TTA→TGA)	acrR →	multidrug efflux transporter transcriptional repressor AcrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	481,482	0	T	G	100.0%	31.5 / NA	9	L89* (TTA→TGA)	acrR	multidrug efflux transporter transcriptional repressor AcrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/4);  total (5/4)

AGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATTAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCGTCGATTATTG  >  NZ_CP009273/481398‑481543                                                                                     |                                                              agaTCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAagag                                                          <  1:197269/90‑1 (MQ=255)       gggAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGatat                                                    <  2:36926/90‑1 (MQ=255)                        aaTATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGaa                                   >  2:41164/1‑90 (MQ=255)                              ggTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACg                             >  1:177586/1‑90 (MQ=255)                              ggTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACg                             >  1:235886/1‑90 (MQ=255)                              ggTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACg                             >  1:88856/1‑90 (MQ=255)                                      agagCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACaga                     <  2:247339/90‑1 (MQ=255)                                             gAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACgg              <  1:6867/90‑1 (MQ=255)                                                         aaaTTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCGTCGATTATTg  >  1:163023/1‑90 (MQ=255)                                                                                     |                                                              AGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATTAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCGTCGATTATTG  >  NZ_CP009273/481398‑481543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑