| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 653,356 | 0 | A | C | 62.5% | 8.0 / 11.9 | 16 | G13G (GGT→GGG) | crcB | fluoride efflux transporter CrcB |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (0/10); total (4/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.24e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATA > NZ_CP009273/653287‑653429 | ccccAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGc > 1:508923/1‑90 (MQ=255) cGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGGCAATAAAAACTGCTAAaag < 1:855419/90‑1 (MQ=255) gAATCGCCTGATGCAGTGGGTAAAATCGCATACTAAACACCCATCTCGCCACGCTCCCCGTCCCACCGCCAATAAAAACTGCTAAaagaa < 2:736009/90‑1 (MQ=255) gTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCaa > 2:779192/1‑90 (MQ=255) ttAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATcc > 1:525014/1‑90 (MQ=255) cATACTAAACAGCCCTCCCCCCCCGCTCCCCGTCCCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTattt < 2:430972/90‑1 (MQ=255) tACTAAACAGCCATCCCCCCACGCTCCCCGTCCCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTatttga < 2:374323/90‑1 (MQ=255) ctaaaCAGCCATCCCGCCCCGTTCCCCGTCCCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTatttgatt < 1:783919/87‑1 (MQ=255) ccATCCCCCCACGCTCCCCTTCCCCCCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAg < 2:835904/90‑1 (MQ=255) aTCTCGCCACGCTTCCCGTCCCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAac < 2:340279/56‑1 (MQ=255) aTCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAac > 1:340279/1‑56 (MQ=255) tcGCCCCGCTCCCCGTCCCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATa < 2:495383/90‑1 (MQ=255) ccgccgcGCTCCCAGAACCCCCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATa < 2:39321/89‑1 (MQ=255) ccGCCCCTCTTCCCTTCCCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATa < 1:226630/89‑1 (MQ=255) ccGCCCCTCTCCCCGCCCCCCCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATa < 1:805567/89‑1 (MQ=255) ccGCCCCGCTCCCCGTCCCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATa < 1:577786/89‑1 (MQ=255) | CCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATA > NZ_CP009273/653287‑653429 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |