| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,141,214 | 0 | T | G | 61.5% | 7.0 / 10.2 | 13 | G20G (GGT→GGG) | yegD | molecular chaperone |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (8/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGA > NZ_CP009273/2141136‑2141297 | ccGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGGGGCGGGAAAACCCAttt > 2:861401/1‑90 (MQ=255) gCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGGCATGCGGGGCGGGAAAACGCATTTTCt > 2:696494/1‑90 (MQ=255) tattTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGGTATGCGTGACGGGAAAACGCATTTTCTAAAAAAGGaa > 2:726738/1‑90 (MQ=255) cGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGGAAAACCCATTTTTTAAAAAATGAAAAAGa > 2:657700/1‑90 (MQ=255) tATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCAc < 2:882095/90‑1 (MQ=255) gTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGGCATGGGTGGCGGGAAAACGCCTTTGTTAAAAAAAGGAAAAGAAAGCACactgc > 1:59272/1‑90 (MQ=255) gTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGc < 1:352475/90‑1 (MQ=255) aaCTGTTCAGTGGCGGTCATGGGTGGCGGGAAACCCCATTTTGTTAAAAAGGAAAAACACAGCCCGCCGCCGCCCTTCATGCCTTtcgcg > 2:432930/1‑90 (MQ=255) cTGTTCAGTGGCGGTCATGCGGGACGGGAAAACGCATTTTCTAAAAAAGGAAAAAAACAACACGCTGCTGCCTTCAATGCTTTGCGCGcc > 2:517296/1‑90 (MQ=255) gTTCAGTGGCGGTCATGCGGGACGGGAAAACCCCTTTTCTAAAAAAGGAAAAAAACAGCACGCCGCCGCCTTCAATGCTTTTCGCGccca > 2:239276/1‑88 (MQ=255) aTGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCCCGCTGTTGCCTTCAATGCTTTGCGCGCGAACGCGTGACGCGGTa < 1:324732/90‑1 (MQ=255) tGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTaa < 2:387308/90‑1 (MQ=255) tGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGa < 2:414882/90‑1 (MQ=255) | CCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGA > NZ_CP009273/2141136‑2141297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |