New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 404679436 (1.170)3 (0.110) 3/138 2.1 7.9% intergenic (+16/+213) hemN/yshB oxygen‑independent coproporphyrinogen III oxidase/YshB family small membrane protein
?NZ_CP009273 = 4046815 38 (1.390)intergenic (+37/+192) hemN/yshB oxygen‑independent coproporphyrinogen III oxidase/YshB family small membrane protein
Rejected: Frequency below/above cutoff threshold.

CGCAACATTTGCATGTGCTTTGATACCTATCTGCGCCAGAAAGCGCGGATGCAGCAGTTCTCTCGGGTGATTTAAATAGTGAATGGCGCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP009273/4046704‑4046794
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atggcgcttTCGTTTTCTACTTTGTAAACGAAGCGCCATTCACTATTTAAATCACCCGAGAGAACTGCTGCATCCGCGCTTTCTGGCGCAGATA  <  NZ_CP009273/4046815‑4046731
                                                                                                                                                                                
CGCAACATTTGCATGTGCTTTGATACCTATCTGCGCCAGAAAGCGCGGATGCAGCAGTTCTCTCGGGTGATTTAAATAGTGAATGGCGCT                                                                                        <  1:1036973/90‑1
                GCTTTGATACCTATCTGCGCCAGAAAGCGCGGATGCAGCAGTTCTCTCGGGTGATTTAAATAGTGAATGGCGCT                                                                                        <  1:62290/74‑1
                GCTTTGATACCTATCTGCGCCAGAAAGCGCGGATGCAGCAGTTCTCTCGGGTGATTTAAATAGTGAATGGCGCT                                                                                        >  2:62290/1‑74
                    TGATACCTATCTGCGCCAGAAAGCGCGGATGCAGCAGTTCTCTCGGGTGATTTAAATAGTGAAT                                                                                              >  1:687379/1‑64
                    TGATACCTATCTGCGCCAGAAAGCGCGGATGCAGCAGTTCTCTCGGGTGATTTAAATAGTGAAT                                                                                              <  2:687379/64‑1
                           TATCTGCGCCAGAAAGCGCGGATGCAGCAGTTCTCTCGGGTGATTTAAATAGTGAATGGCGCTTTCGTTTTCTACTTTGTAAACGAAG                                                               <  1:664948/88‑1
                           TATCTGCGCCAGAAAGCGCGGATGCAGCAGTTCTCTCGGGTGATTTAAATAGTGAATGGCGCTTTCGTTTTCTACTTTGTAAACGAAG                                                               >  2:664948/1‑88
                                                                              GTGAATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTCACTATTTAAATCACCCGAGAGAACTGCTGCATCCGCGCTTTCTGG          >  1:992650/1‑90
                                                                                  ATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTCACTATTTAAATCACCCGAGAGAACTGCTGCATCCGCGCTTTCTGGCGCA      <  1:840051/90‑1
                                                                                      CGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTCACTATTTAAATCACCCGAGAGAACTGCTGCATCCGCGCTTTCTGGCGCAGATA  <  2:580812/90‑1
                                                                                                                                                                                
CGCAACATTTGCATGTGCTTTGATACCTATCTGCGCCAGAAAGCGCGGATGCAGCAGTTCTCTCGGGTGATTTAAATAGTGAATGGCGCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP009273/4046704‑4046794
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atggcgcttTCGTTTTCTACTTTGTAAACGAAGCGCCATTCACTATTTAAATCACCCGAGAGAACTGCTGCATCCGCGCTTTCTGGCGCAGATA  <  NZ_CP009273/4046815‑4046731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.