| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 510,067 | 0 | C | T | 57.1% | ‑0.6 / 15.1 | 14 | K83K (AAG→AAA) | ybbJ | NfeD family protein |
| Reads supporting (aligned to +/- strand): ref base C (6/0); new base T (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCA > NZ_CP009273/509981‑510153 | ccGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCttt > 1:918284/1‑90 (MQ=255) cgggggAGATCCCAGCAAAAATCGCCGGCAAACCGGCGGCCGCCCGCGCTGGTTTAAATGACTATCGCTGTGTTTTTGTTCGCCCCCccg < 1:844270/86‑1 (MQ=255) ggCGGAGATCCCAGCACAAACCGTCGCCCAATCACCCCCCCCCCGCGTTGTTTAAAATGACTATCGTTGTGTTTTTGTTCGCCCCCccgc < 2:918284/89‑1 (MQ=255) gCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACccgcc > 2:844270/1‑90 (MQ=255) gaTTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGAc > 1:743282/1‑90 (MQ=255) gaTCCCAGAAAAAACCGCCGGCCAATCGGCTGCCCCCCGCGCTGTTTTAAATGACTATCGCTGTGTTTTTGTTCGCCCCCCCGCCGCGAc < 1:843983/90‑1 (MQ=255) cAGCACAAACCGTCGGCAAATCAGCTGCCCCCCGCGCTGGTTTAAATGCCTACCGCTGTGTTTTTGTCCGCGCCCCCGCCGCGACAACCa < 1:337637/90‑1 (MQ=255) cAGCAAAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGTTTAAAATGACTATCGCTGTGTTTTTGTTCGCGCCCCCGCCGCGACAACCa < 1:399894/90‑1 (MQ=255) tCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAg > 1:67398/1‑90 (MQ=255) gCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCa > 1:883875/1‑90 (MQ=255) ttaaaaTGCCTCTCGCTGTTTTTTTGTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCa < 2:157871/87‑1 (MQ=255) ttaaaaTGCCTAGCGCTGTGTTTTTGTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCa < 2:795462/87‑1 (MQ=255) ttaaaaTGCCTACCGTTGTTTTTTTGTCCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCa < 2:524277/87‑1 (MQ=255) gtgtTTTTGTGCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCa < 2:856596/90‑1 (MQ=255) gtgCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCa > 1:856596/1‑90 (MQ=255) | CCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCA > NZ_CP009273/509981‑510153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |