| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 642,203 | 0 | T | G | 53.3% | 1.4 / 18.8 | 15 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase CitG |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (8/0); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTAT > NZ_CP009273/642124‑642292 | tCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGccc > 1:777030/1‑90 (MQ=255) gCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGGAGGcccccc > 2:537781/1‑89 (MQ=255) cgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTcg < 2:777030/90‑1 (MQ=255) gATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGGTGGATGGCCCCCTTTTTTTATAATTTTTTCGCCTCCCGCTg > 2:583403/1‑90 (MQ=255) cccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGCATTCCCCCCTTTTTTAATAATGTTTTCGCCCCCCCCTGTAGCCAgg > 1:834950/1‑89 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGAAGCCCCCCTTTTTCAATAAAATTTTTGCCCCGCCCCGTTGCCAgcgcc > 2:802206/1‑89 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATTCCCCCCTTTTTTTATAAATTTTTTGCCCCCCGCCGTAGCCCgcgcc > 2:773364/1‑89 (MQ=255) cGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGc < 2:102444/90‑1 (MQ=255) cGGAGATAATCGAGATCGGCGGGGGGTTGAATGCCCCCCTTTTTCAATAATGTTTTCGCCTCCCGCTGTAGCCCGCGCCGGccccccccc > 1:102444/1‑87 (MQ=255) aaTCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgc < 2:275300/90‑1 (MQ=255) aTCGAGATCGGCGGGGGGTTGTAAACCCCCCTTTTTCCATAATGTTTTCGCCCCCCCCTGTCGCCAAGCcccgccccccccgcccccccc > 1:444527/1‑87 (MQ=255) tCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcgc < 1:537781/90‑1 (MQ=255) tCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcgc < 2:834950/90‑1 (MQ=255) aTCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCCCCGCGCGATGc < 2:444527/90‑1 (MQ=255) gTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTa < 1:703549/90‑1 (MQ=255) ttCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTAt < 2:640701/90‑1 (MQ=255) | TCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTAT > NZ_CP009273/642124‑642292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |