| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 760,187 | 0 | G | C | 53.3% | 10.2 / 23.1 | 15 | P184P (CCG→CCC) | sucD | succinate‑‑CoA ligase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (0/7); new base C (8/0); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCT‑CGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGC > NZ_CP009273/760103‑760276 | acTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGctc < 2:797047/90‑1 (MQ=255) gACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGGCCCACCCCCGGctctta > 2:741701/1‑88 (MQ=255) gACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGCCCCGTCCCCGGCTCTaa > 1:509050/1‑90 (MQ=255) cTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGCCCCCACCCCGGCCCTCACtt > 2:1000665/1‑90 (MQ=255) ggTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCCGGCTCTAACTTTTTTGACAt > 1:67229/1‑90 (MQ=255) ccACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCCATCCCCGGCTCTAACTTTTTTCACATTTTCCGAAAtt > 1:414985/1‑89 (MQ=255) cGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCg < 2:955787/56‑1 (MQ=255) cGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCCATCCCg > 1:955787/1‑56 (MQ=255) gATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCT‑CGAAATGTTCGaa < 2:631396/90‑1 (MQ=255) ggTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGCTCCCCGGCTCTAACTTTTTCGACAATCT‑CGAAATGTTTGAAAAAAAACCCCAg > 1:804856/1‑90 (MQ=255) cAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCT‑CGAAATGTTCGAAAAAGATCCGCAGAcc < 1:775135/90‑1 (MQ=255) gACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCT‑CGAAATGTTCGAAAAAGATCCGCAGACCGAAGc < 1:1000665/90‑1 (MQ=255) gACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCT‑CGAAATGTTCGAAAAAGATCCGCAGACCGAAGc < 1:622491/90‑1 (MQ=255) gtCGGTATCGGCGGTGACCCCATCCCCGGGCCTAACTTTTTTTACATTCT‑CCCAATGTTTGAAAAAAAAACGCAGACCGAAGCGGTCggg > 2:325022/1‑88 (MQ=255) cggcggTGACCCGATCCCCGGCTCTAACTTTTTCGACATTCT‑CGAAATGTTTGAAAAAAATCCGCAGACCGAAGCGATCGTGGTGATCgg > 1:342981/1‑90 (MQ=255) cggTGACCCGATCCCGGGCTCTAACTTTATCGACATTCT‑CGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTga < 1:857152/90‑1 (MQ=255) aTCCCGGGCTCTAACTTTATCGACATTCT‑CGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGt < 1:64462/90‑1 (MQ=255) gggCTCTAACTTTATCGACATTCT‑CGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAgcgc < 2:509050/90‑1 (MQ=255) gggCTCTAACTTTATCGACATTCT‑CGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAgcgc < 1:741701/90‑1 (MQ=255) | ACTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCT‑CGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGC > NZ_CP009273/760103‑760276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |