| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,837,171 | 0 | A | G | 53.3% | 0.3 / 16.7 | 15 | M182V (ATG→GTG) | gdhA | NADP‑specific glutamate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (0/7); new base G (8/0); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTC‑TTCA‑CCGGTAAGGGCCTTTCATT > NZ_CP009273/1837092‑1837229 | tCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGaaaaa < 2:532870/90‑1 (MQ=255) aCCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGctct < 2:234389/90‑1 (MQ=255) cTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGATGGAAAAAATCTcc > 1:251562/1‑90 (MQ=255) cTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGATGAAAAAACTCTcc > 1:322160/1‑90 (MQ=255) gggCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGGTGAAAAAAATCTccca > 1:288720/1‑88 (MQ=255) aCCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGggtg > 1:211234/1‑63 (MQ=255) aCCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGgatg < 2:211234/63‑1 (MQ=255) gTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTc < 2:540370/90‑1 (MQ=255) gTTCCGGCAGGTGATATAGGGGTTGGTGTTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTc < 2:1018054/90‑1 (MQ=255) ggCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTC‑TTCA‑c < 1:264354/90‑1 (MQ=255) ggCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTC‑TTCA‑c < 2:322160/90‑1 (MQ=255) gCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTC‑TTCA‑cc < 1:52664/90‑1 (MQ=255) tatCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGGTGAAAAAAAACTCCCACAAAAACCCCCTCGTC‑TTCACCCGGTAAgg > 2:572865/1‑90 (MQ=255) ggggTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGGTGAAAAAAATCTCCCACAAAAACCCCCGCGTC‑TTCC‑CCCGGAAGGGCCtt > 1:222633/1‑90 (MQ=255) gggTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGAGGAAAAAAAACTCCCACAAAAACCCCCGCGTCTTTCC‑CCGGAAAGGGCCtt > 1:550597/1‑90 (MQ=255) tggtggTCGTGAAGTCGGCTTTATGGCGGGGGTGATGAAAAAAGTCTCCAACAAAACCGCCCGCGTC‑TTCA‑CCGGGAAGGGGCCTTTAtt > 2:336766/1‑90 (MQ=255) tggtggTCGTGAAGTCGGCTTTATGGCGGGGGTGATGAAAAAAATCTCCCAAAAAACCCCCCGCGGC‑TTTT‑CCCGGAAGGGGCCTTTAtt > 1:256446/1‑90 (MQ=255) | TCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTC‑TTCA‑CCGGTAAGGGCCTTTCATT > NZ_CP009273/1837092‑1837229 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |