| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,126,661 | 0 | T | G | 60.0% | ‑0.4 / 15.8 | 15 | H219P (CAC→CCC) | yghS | dTMP kinase |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (9/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCA‑GTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTA > NZ_CP009273/3126583‑3126733 | tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc < 1:341688/90‑1 (MQ=255)tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc < 1:770279/90‑1 (MQ=255)tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc < 1:883784/90‑1 (MQ=255)tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc < 2:37086/90‑1 (MQ=255)tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc < 2:736504/90‑1 (MQ=255)tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGGGCCCCTcc > 2:11791/1‑90 (MQ=255) cccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCA‑g < 2:403986/90‑1 (MQ=255) aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgtg < 1:898409/74‑1 (MQ=255) aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgtg > 2:898409/1‑74 (MQ=255) aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCC‑GGTCa > 1:905157/1‑90 (MQ=255) gcggcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgtg < 2:169395/50‑1 (MQ=255) gcggcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgtg > 1:169395/1‑50 (MQ=255) gcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCA‑ATTCAAGGAACTTTTGGCCCTTTAATGGCaat > 2:402965/1‑88 (MQ=255) gAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCC‑GTTCAAGGATCTTTTCGCCCTTTAATGTTAGTTggg > 2:266378/1‑88 (MQ=255) gcgcGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCCCCC‑CTTTAAAGGGCTTTTTGCCCTTTAATGGCAGTTgggggggg > 2:969391/1‑86 (MQ=255) cgcgTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGTGCCCCCCCACGTTCAA‑GAACTTTTTTCCCTTGTATGTGTGTTGTGGCGTaa > 2:939875/1‑90 (MQ=255) cgcgTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCC‑GTTTAAGGGGCTTTTTGGCCTTTTATGTTAGTTGGGGCGtga > 2:978881/1‑88 (MQ=255) cTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCA‑GTTCAAGGGTCTTTTCGCCATTGAATGTCAGTTGCGGCGGAACGGGGAgttttt > 2:17093/1‑90 (MQ=255) gAATTTCATCGGCGGGGGGGTGCCCATCCC‑GGTTAAGGGTCTTTTCGCCCTTTGATGTCAGTTGGGGGGGAAAGGCGGTTTTTTTCtttt > 2:832951/1‑87 (MQ=255) | TCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCA‑GTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTA > NZ_CP009273/3126583‑3126733 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |