| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,199,811 | 0 | C | A | 45.5% | 32.2 / 11.9 | 22 | intergenic (‑196/‑11) | ttdR/ttdA | DNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (1/11); new base A (10/0); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTA > NZ_CP009273/3199728‑3199879 | aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGt < 2:432423/90‑1 (MQ=255)aCAGATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGt < 1:50981/90‑1 (MQ=255) cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATgggggt > 1:73675/1‑86 (MQ=255) gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCCtgatga > 1:261159/1‑90 (MQ=255) ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAgg > 1:8027/1‑89 (MQ=255) gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAGACAAAAAAAAAAAATGGGGGTTGCatgatgctgt > 2:50981/1‑86 (MQ=255) aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaa < 2:304874/90‑1 (MQ=255) atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaa < 1:33203/90‑1 (MQ=255) ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaata < 2:607824/90‑1 (MQ=255) acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGGGTTGCCCTGATGAGCGAAAAGAAAAAacaacaa > 1:607824/1‑89 (MQ=255) acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGGGAAAGTAATAAACAACAg > 1:69078/1‑90 (MQ=255) acaTTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCAGACAAAAAAAAAAAATGGGGTTGCCATTATGGGGGGAAAGAATAAACAAAAg > 1:867324/1‑90 (MQ=255) aTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc < 2:60884/90‑1 (MQ=255) aTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc < 2:888580/90‑1 (MQ=255) tAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGa < 2:8027/90‑1 (MQ=255) aGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGGAAGGAAAAAACAAAAAGCAgggga > 1:476507/1‑86 (MQ=255) ggTAGTTTGTGGCGGGGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGGCATGATGAGGGAAAAGAATAAGGAAAAAGCAGGGGATaa > 1:852421/1‑90 (MQ=255) ggTAGTTTGTGGCGGGGAAATTTTTCAGACAAAAAAAAAAAATGGGGGTGCCCTGATGGGGGGAAGTAATAAACAAAAGGAAATGAaaaa > 2:653564/1‑90 (MQ=255) tgtgGGGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa < 1:626547/90‑1 (MQ=255) ttttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa < 2:73675/90‑1 (MQ=255) aaCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa > 2:193797/1‑71 (MQ=255) aaCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa < 1:193797/71‑1 (MQ=255) | ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTA > NZ_CP009273/3199728‑3199879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |