| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,234,344 | 0 | T | G | 46.3% | 47.7 / 16.5 | 41 | S348A (TCT→GCT) | sstT | serine/threonine transporter SstT |
| Reads supporting (aligned to +/- strand): ref base T (5/17); new base G (19/0); total (24/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.15e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATC > NZ_CP009273/3234255‑3234430 | tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGt > 2:68055/1‑90 (MQ=255) tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTctgct > 1:498424/1‑90 (MQ=255) gTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTctgctgctg < 2:337381/90‑1 (MQ=255) gTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTctgctgctg < 2:498424/90‑1 (MQ=255) gTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTctgctgctg > 2:278205/1‑90 (MQ=255) gTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTctgctgctg > 1:337381/1‑90 (MQ=255) gTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGGGGCGCATCCGGCGTGGCGGGGGGGGCCctgcggctg > 2:495411/1‑90 (MQ=255) cGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGAt > 2:160919/1‑90 (MQ=255) aTCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATcc > 1:263663/1‑90 (MQ=255) cccACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTg < 1:160919/90‑1 (MQ=255) aCGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGcc < 2:263663/90‑1 (MQ=255) aCGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGcc < 2:382590/90‑1 (MQ=255) aCGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGcc < 1:807091/90‑1 (MQ=255) aCGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGcc < 1:68055/90‑1 (MQ=255) cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCCCTTGCCt > 1:862000/1‑90 (MQ=255) ctgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGtc < 2:857903/62‑1 (MQ=255) ctgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGtc < 1:76029/62‑1 (MQ=255) ctgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGtc > 1:857903/1‑62 (MQ=255) ctgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGcc < 2:931355/84‑1 (MQ=255) ctgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGcc > 1:931355/1‑84 (MQ=255) tGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCAc < 2:811021/72‑1 (MQ=255) tGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCAc < 2:423269/72‑1 (MQ=255) tGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCAc > 1:811021/1‑72 (MQ=255) tGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCAc > 1:423269/1‑72 (MQ=255) gAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCgg < 2:833162/90‑1 (MQ=255) gCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCCCTGGCCTGTAATATGTTTggga > 1:497778/1‑88 (MQ=255) gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCAc > 1:811492/1‑67 (MQ=255) gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCAc < 2:811492/67‑1 (MQ=255) gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGTCTGCTGCTGATCCCCCCGGCCTGTTATATGTTTggggtt > 2:720821/1‑86 (MQ=255) gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGTTTCCCCCGGCCTGTAATATGTTCggggtt > 2:883122/1‑86 (MQ=255) gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTTGGGAtt > 1:721302/1‑90 (MQ=255) gtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGtc < 1:378640/47‑1 (MQ=255) gtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGtc < 1:1013962/47‑1 (MQ=255) gtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCCCTGGCCTGTAATATGTTTGGGATTTCg > 2:189051/1‑90 (MQ=255) tggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGa < 1:883122/90‑1 (MQ=255) ttCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCCCTGGCCTGTAATATGTTCGGGATTTTGAACGa > 2:258891/1‑90 (MQ=255) tgtgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGGATTTCGAACGATATCg > 1:714421/1‑90 (MQ=255) tgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGcc < 2:666454/57‑1 (MQ=255) tgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGcc < 1:647762/57‑1 (MQ=255) tgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCCCTGGcc > 2:647762/1‑57 (MQ=255) tgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGcc > 1:666454/1‑57 (MQ=255) gtgGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAgg > 1:952645/1‑90 (MQ=255) ggCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtg > 1:426083/1‑90 (MQ=255) cgcATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGGATTTCGAACGATATCGCCATGCAggtggt > 1:419344/1‑90 (MQ=255) aTCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTTTAATATGTTCGGGATTTTGAACGATATCGCCCTGCAGGGGGGTGc > 2:122816/1‑90 (MQ=255) ggggggCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGGGGGTGCCGTCGGCTTTttca > 1:139466/1‑90 (MQ=255) gggTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcatc < 2:524416/90‑1 (MQ=255) gggTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcatc < 2:95453/90‑1 (MQ=255) | TGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATC > NZ_CP009273/3234255‑3234430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |