| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,958 | 0 | G | A | 54.5% | 6.2 / 25.6 | 22 | L57L (CTG→TTG) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (7/3); new base A (0/12); total (7/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGT > NZ_CP009273/4058870‑4059027 | aaTTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGa < 1:599589/90‑1 (MQ=255) cccaaaTTTTTTCCCGGGAGTCGAAAAAAAACCCGGTGAGCATATCGGAAAACGCGGAAAAAACCTCCCCCCCAAAAAAAATAACCCCGc < 2:146719/86‑1 (MQ=255) ttttCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAg > 1:456580/1‑90 (MQ=255) tttCGGCGAGTCGAAAAAAAACCGGGTGACCATACGGGAAAACGCGGAAAAAACCTCCCCCACAAAAAAGATAACCCCGCCATAGTAAGc < 2:910227/90‑1 (MQ=255) tttCCGCGAGCCAAAAAAAAATCGGGTGACCATACGGGAAAACGCGAAAAAAACCTCCCCCACCAAAAAAATACTCCCGCCATAGTAAGc < 2:703764/90‑1 (MQ=255) cgcgAGTCGAGAAAAAATCGGGGGAGCATCTGGGAAAACGGGGTAAAAACCTCCGCCACCAGAAAAAAAATCCCGCCATAGTAAGCAGGc < 1:896963/90‑1 (MQ=255) cgcgAGTCGAGAAAAAACCCGGTGAGCATATCGGTAAACGCGGAAAAAAACTTCCCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGc < 1:295553/90‑1 (MQ=255) gcgAGTGGAAAAAAAACCCGGGGAGCATATCGGAAAACGCGGAAAAAAACTCCGCCACAAAAAAGATAACCCCGCCATAGTAAGCAGGCa < 1:619548/90‑1 (MQ=255) gcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCa > 1:520729/1‑90 (MQ=255) gcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCa > 1:530998/1‑90 (MQ=255) gcgAGTAGAGAAAAAACCGGGGGAGCATATCGGAAAACGGGAAAAAAACCTTCCCCACCAAAAAAAAAACCCCGCCATAGTAAGCAGGCa < 2:987909/90‑1 (MQ=255) aaaTCGGGTGAGAATATCGGAAAACGCGAAAAAAACCTCCCCCACAAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 2:530998/90‑1 (MQ=255) aaaTCCGGTGAGCATATCGGTAAACGCGGAAAAAAACTTCCCCCCCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 1:328850/90‑1 (MQ=255) aaTCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg > 1:299593/1‑90 (MQ=255) ggTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata > 2:614409/1‑90 (MQ=255) ggTGAGCATATCGGAAACCGGGGAAAAAAACTTCCCCCCAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata < 2:388625/90‑1 (MQ=255) tagcGGAAAACGGGATAAAAACCTCCGCCACAAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTga < 1:497686/87‑1 (MQ=255) gTAACCGCGAAAAAAACCTTCCCCACCAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGgaga < 1:471761/90‑1 (MQ=255) aaaCGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATa > 2:593118/1‑90 (MQ=255) aaCGCGAAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATAc < 2:913185/90‑1 (MQ=255) gcgGAAAAAAACTTCGCCACAAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACaga < 1:342689/90‑1 (MQ=255) ggTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGt > 1:913185/1‑90 (MQ=255) | AATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGT > NZ_CP009273/4058870‑4059027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |