| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,213,865 | 0 | G | T | 57.1% | 9.0 / 30.2 | 21 | S361I (AGT→ATT) | pdeG | cyclic diguanylate phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (8/1); new base T (0/12); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.42e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.95e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTT > NZ_CP009273/1213805‑1213949 | ttAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTc > 1:651888/1‑90 (MQ=255) gcTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGaa > 2:337149/1‑90 (MQ=255) cTTCGTATATTATTTCGCCGACTTTTGTCGACGGGTTTTTAAATTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAg < 1:759770/90‑1 (MQ=255) ttCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGt > 1:492571/1‑90 (MQ=255) ttCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGt > 1:92701/1‑90 (MQ=255) atatTTTTTCGCCGACTTTTGTCGAGGAGTTTTTAATTTTCCGTGAAGGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCg < 2:418921/90‑1 (MQ=255) tattTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCg > 2:1132085/1‑90 (MQ=255) ccGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCa > 2:757109/1‑90 (MQ=255) gtTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTc > 1:400309/1‑90 (MQ=255) gtTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTc > 1:716057/1‑90 (MQ=255) tttAATTTTCCGTGACATTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCt < 1:122504/90‑1 (MQ=255) tttCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCa < 1:989637/90‑1 (MQ=255) ttcccGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCa < 1:300376/87‑1 (MQ=255) ttcccGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCa < 2:465789/87‑1 (MQ=255) tCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 2:940445/90‑1 (MQ=255) tCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 2:712874/90‑1 (MQ=255) cGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCgg < 2:445744/90‑1 (MQ=255) cGTGACATTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCgg < 2:796034/90‑1 (MQ=255) tcaCATTTTCACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGtt < 2:327343/88‑1 (MQ=255) tGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGtt < 2:354889/90‑1 (MQ=255) tGACATTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGtt < 2:244449/90‑1 (MQ=255) | TTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTT > NZ_CP009273/1213805‑1213949 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |